CAREER: Development of a point-of-care platform for the detection of infectious viruses

职业：开发用于检测传染性病毒的即时护理平台

• 批准号: 1942487
• 负责人: Waseem Asghar
• 金额: $ 50万
• 依托单位: Florida Atlantic University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1942487&HistoricalAwards=false
• 关键词: CAREER; Development; point; care; platform

Every year, infectious viruses cause millions of deaths worldwide. Current technologies for testing virus infections often require multiple labor-intensive steps and need costly and bulky equipment, hence are not suitable for rapid disease testing at point-of-care (POC) settings. The goal of this CAREER project is to develop a sample-in-answer-out technology for rapid detection of multiple viruses simultaneously with minimal sample preparation at POC settings. It aims to produce a low-cost, disposable device that integrates fully automated assay with smartphone-based readout for reporting and surveillance of current and emerging infectious diseases. This project integrates multidisciplinary research with educational activities to engage high school teachers and students at different levels (K-12, undergraduate and graduate students). It helps to prepare a skilled workforce and broaden participation in STEM fields.Through combined scientific research and device engineering, the Asghar group at Florida Atlantic University aims to develop a low-cost, functional device that can be widely deployed for POC diagnosis of viral infections. The research team explores the use of water-oil interphase separation in a microfluidic device integrated with magnetic actuation platform and smartphone-based readout to allow automation and improve throughout. The approaches to enabling analysis of complex samples (including blood, saliva and urine) with minimal sample preparation and resources include employing reverse transcription loop-mediated isothermal amplification (RT-LAMP) and using synthetic "self-avoiding" nucleotide analogs to address the current limitations of primer dimerization.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

每年，传染性病毒在全世界造成数百万人死亡。目前检测病毒感染的技术通常需要多个劳动密集型步骤，并且需要昂贵和笨重的设备，因此不适合在医疗点（POC）环境中进行快速疾病检测。本CAREER项目的目标是开发一种样品-答案-输出技术，在POC设置下，以最少的样品制备同时快速检测多种病毒。它的目标是生产一种低成本的一次性设备，将全自动检测与基于智能手机的读数相结合，用于报告和监测当前和新出现的传染病。该项目将多学科研究与教育活动结合起来，吸引不同层次的高中教师和学生（K-12，本科生和研究生）。它有助于培养熟练的劳动力，并扩大STEM领域的参与。通过科学研究和设备工程的结合，佛罗里达大西洋大学的Asghar小组旨在开发一种低成本、功能齐全的设备，可以广泛用于POC病毒感染的诊断。研究小组探索了在微流体设备中使用水-油界面分离，该设备集成了磁驱动平台和基于智能手机的读数，以实现自动化和改进整个过程。用最少的样品制备和资源分析复杂样品（包括血液、唾液和尿液）的方法包括使用逆转录环介导的等温扩增（RT-LAMP）和使用合成的“自我避免”核苷酸类似物来解决引物二聚化的局限性。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Diagnosis for COVID-19: current status and future prospects

• DOI: 10.1080/14737159.2021.1894930
• 发表时间: 2021-02
• 期刊: Expert Review of Molecular Diagnostics
• 影响因子: 5.1
• 作者: Alamgir Kabir;R. Ahmed;S. M. A. Iqbal;R. Chowdhury;R. Paulmurugan;U. Demirci;W. Asghar