PFI-TT: Development of Point-of-Care Biochips for the Rapid and Sensitive Detection of Multiple Respiratory Pathogens

PFI-TT：开发用于快速、灵敏检测多种呼吸道病原体的床旁生物芯片

基本信息

批准号：
2122712
负责人：
XiuJun Li
金额：
$ 25万
依托单位：
University of Texas at El Paso
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2122712&HistoricalAwards=false
关键词：
PFI TT Development Point Care

项目摘要

The broader impact/commercial potential of this Partnerships for Innovation - Technology Translation (PFI-TT) project stems from the critical need for a low-cost, point-of-care (POC) microfluidic device for the rapid detection of multiple respiratory pathogens (such as whooping cough and pneumonia) in vulnerable people in low-resource settings. For instance, there are an estimated 24.1 million cases of pertussis worldwide, and about 160,700 deaths per year from whooping cough in children. The new microfluidic device can provide low-cost, rapid, and accurate detection of multiple respiratory pathogens at the point of care, enabling detection of whooping cough and pneumococcal pneumonia within one hour at various venues such as physicians’ offices, schools, rural areas, and developing nations. Rapid pathogen detection at the point of care (POC) can significantly reduce mortality in children and the elderly. The microfluidic device also has the potential to detect a variety of other pathogens such as foodborne pathogens and SARS-CoV-2. Hence, the societal and commercial impacts of the translational development of this technique may impact global health. This project is primarily intended to develop a low-cost microfluidic device prototype integrated with specific and sensitive DNA testing techniques for the rapid and accurate detection of multiple common respiratory pathogens (i.e., B. pertussis and S. pneumonia)in vulnerable people at the point of care. Conventional pathogen detection methods either take a long time or require costly and bulky instruments, limiting their applications for low-resource settings. This approach is based on integrated loop-mediated isothermal amplification (LAMP) on a low-cost paper/polymer hybrid microfluidic device. DNA amplification and six primers specific to each pathogen target ensure high detection sensitivity and high accuracy, respectively, while the instrument-free detection by the naked eye minimizes instrumentation requirements. The integration of LAMP on a portable microfluidic device further enhances its capability for POC testing.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这种合作伙伴关系对创新 - 技术翻译（PFI-TT）的更广泛的影响/商业潜力，从对低估的人群中低成本的低成本，护理（POC）微流体设备的关键需求，用于快速检测多种呼吸道病原体（例如，在低估的设置中，在脆弱的人群中快速检测到多种呼吸病原体（例如，咳嗽和肺炎）。例如，估计有2410万例全球百日咳病例，每年大约有160,700例儿童咳嗽死亡。新的微流体设备可以在护理点上提供低成本，快速和准确检测多种呼吸道病原体，从而在一小时内在一小时内在各个场所（例如医生的办公室，学校，艰难地区和发展中国家）在一小时内检测百日咳和肺炎球菌肺炎。在护理点（POC）处的病原体检测可以显着降低儿童和年龄较大的死亡率。还具有检测各种其他病原体（例如食源性病原体和SARS-COV-2）的潜力。因此，该技术的翻译发展的社会和商业影响可能会影响全球健康。该项目主要旨在开发一种与特定和敏感的DNA测试技术集成的低成本微流体设备原型，以快速，准确地检测在较脆弱的人中，对多种常见的呼吸道病原体（即B. terpussis and S. pneumonia）进行了多种常见的呼吸病原体（即B. terpussis and S. pneumonia）。传统的病原体检测方法要么需要很长时间，要么需要昂贵且笨重的仪器，从而限制了其用于低资源设置的应用。这种方法基于低成本纸/聚合物混合微流体设备上的集成环介导的等温扩增（LAMP）。 DNA扩增和针对每个病原体靶标的六个引物可确保高度检测灵敏度和高精度，而肉眼的无仪器检测可最大程度地减少仪器的要求。灯在便携式微流体设备上的集成进一步增强了其在POC测试中的能力。该奖项反映了NSF的法定任务，并使用基金会的知识分子优点和更广泛的影响审查标准，被视为通过评估来获得珍贵的支持。