Point-of-care Biosensing Using a Novel Paper Fluidic and SERRS

使用新型纸质流体和 SERRS 进行即时生物传感

• 批准号: 2022805
• 负责人: Gerard Cote
• 金额: $ 54万
• 依托单位: Texas A&M Engineering Experiment Station
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2022805&HistoricalAwards=false
• 关键词: Point; care; Biosensing; Using; Novel

Present health monitoring and diagnosis devices are typically limited to bulky, often complicated, and costly equipment accessible only in a central laboratory. This research project aims to develop a low cost, high quality system for monitoring biomarkers of diseases (such as cardiac disorders, cancer, viral infections, and preeclampsia) in clinical as well as in remote and low resource settings. Preeclampsia is the initial target as it is a leading cause of maternal and fetal mortality and morbidity, but no preventative test is currently available. A low-cost, reliable technique for detecting biomarkers of preeclampsia provides the opportunity for early intervention before symptoms occur and enables closer monitoring of women at increased risk. This project provides research training to diverse students at the graduate and undergraduate level. The research team works with the university programs, including Society of Mexican American Engineers and Scientists, National Society of Black Engineers, and the Society of Women Engineers and Women's Research Symposium, to broaden participation in science and engineering. In addition, the team engages high school students and teachers in university-based laboratory research to excite them about science and engineering. The point-of-care diagnostic system being developed uses a paper-based microfluidics cartridge that contains novel metal nanoparticles functionalized with Raman reporters and biomarker probes. The change in surface-enhanced resonance Raman scattering (SERRS) signal in the presence of target biomarkers in a blood sample is read by a handheld Raman spectrometer. The specific aims of this project are: 1) the design and study of novel metal nanoparticles functionalized with Raman reporter molecules to understand the structural factors that influence the surface-enhanced resonance Raman scattering signal; 2) the development of bioassays to monitor the preeclampsia biomarkers with high sensitivity and specificity; and 3) the design and development of the three dimensional multichannel paper microfluidic cartridge for simultaneous monitoring of multiple early epigenetic biomarkers.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

目前的健康监测和诊断设备通常仅限于只有在中心实验室才能使用的笨重、通常复杂和昂贵的设备。该研究项目旨在开发一种低成本、高质量的系统，用于在临床以及偏远和低资源环境中监测疾病(如心脏疾病、癌症、病毒感染和先兆子痫)的生物标志物。先兆子痫是最初的目标，因为它是孕产妇和胎儿死亡和发病率的主要原因，但目前还没有预防性测试。一种低成本、可靠的检测先兆子痫生物标志物的技术提供了在症状出现之前进行早期干预的机会，并使更密切地监测风险增加的妇女成为可能。该项目为不同的研究生和本科生提供研究培训。研究团队与大学项目合作，包括墨西哥美国工程师和科学家协会、全国黑人工程师协会和女性工程师协会和女性研究研讨会，以扩大对科学和工程的参与。此外，该团队还邀请高中生和教师参与基于大学的实验室研究，以激发他们对科学和工程的兴趣。正在开发的护理点诊断系统使用一种纸基微流控墨盒，其中包含由拉曼报告和生物标记探针功能化的新型金属纳米颗粒。通过手持式拉曼光谱仪读取血液样本中目标生物标志物存在时表面增强共振拉曼散射(SERRS)信号的变化。该项目的具体目标是：1)设计和研究具有拉曼报告分子功能的新型金属纳米颗粒，以了解影响表面增强共振拉曼散射信号的结构因素；2)开发高灵敏度和特异性的生物检测方法，以监测先兆子痫生物标志物；以及3)设计和开发同时监测多个早期表观遗传生物标志物的三维多通道纸质微流控芯片。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。