Collaborative Research: GCR: Infection-Resisting Resorbable Scaffolds for Engineering Human Tissue

合作研究：GCR：用于工程人体组织的抗感染可吸收支架

• 批准号: 2219014
• 负责人: Matthew Libera
• 金额: $ 132.5万
• 依托单位: Stevens Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-10-01 至 2027-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2219014&HistoricalAwards=false
• 关键词: Collaborative; Research; GCR; Infection; Resisting

Tissue engineering is a thought-provoking concept made compelling by the need to replace failed, damaged, or defective body parts. Unlike the more mature field of traditional medical-device development (e.g., joint replacement), tissue engineering is young and evolving. This project can thus change the established tissue-engineering paradigm to not only address the development of healthy tissue (associated with healing) but simultaneously address the need to inhibit bacterial colonization (associated with infection). A convergent team of researchers with expertise in microbiology, polymer science, biomaterials science, computational chemistry, veterinary medicine, and medical-device development will develop a new and flexible approach to infection control within a resorbable scaffold for tissue engineering. The research plan encorporates three synergistic research thrusts which will converge around a specific testbed, namely a new scaffold designed to regrow hard tissue that simultaneously resists bacterial colonization. To mimic the extracellular matrix, Thrust 1 will exploit 3D printing to additively create fiber-based scaffolds with controllable fiber size/spacing using combinations of resorbable polymers together with signaling factors to influence stem-cell differentiation. Thrust 2, using synergistic computational and experimental approaches, will explore fundamental concepts of polyelectrolyte complexation and directed self-assembly to render these scaffolds self-defensive (able to release antimicrobials only if, when, and where there is a bacterial challenge). Thrust 3 will employ lab-on-a-chip concepts to understand the competition between mammalian cells and bacteria and, in Phase 2 of project, include small-animal infection models able to recapitulate a complex physiological response.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

组织工程是一个发人深省的概念，这是由于需要替换失败，损坏或有缺陷的身体部位而引人注目。与传统医疗设备开发的更成熟的领域（例如关节置换）不同，组织工程年轻且不断发展。因此，该项目可以改变已建立的组织工程范式，不仅解决了健康组织的发展（与愈合有关），而且还解决了抑制细菌定植（与感染相关的）的需求。具有微生物学专业知识，聚合物科学，生物材料科学，计算化学，兽医医学和医疗设备开发方面的专业知识的研究人员团队将开发一种新的灵活方法，以在组织工程的可吸收支架内进行感染控制。该研究计划包含了三项协同研究推力，这些研究将围绕特定的测试床汇聚，即一种新的脚手架，旨在再生硬组织，同时抵抗细菌定植。为了模仿细胞外矩阵，推力1将利用可吸收聚合物的组合以及信号因子以及影响干细胞分化的，使用可控的纤维尺寸/间隔来添加3D打印以创建具有可控纤维尺寸/间距的基于纤维的支架。推力2，使用协同计算和实验方法，将探索聚电解质络合的基本概念，并定向自组装以使这些脚手架自我防御（只有在何时，何时何时何地释放出有细菌挑战的地方））。 Thrust 3 will employ lab-on-a-chip concepts to understand the competition between mammalian cells and bacteria and, in Phase 2 of project, include small-animal infection models able to recapitulate a complex physiological response.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

项目成果

Self-Defensive Antimicrobial Surfaces Using Polymyxin-Loaded Poly(styrene sulfonate) Microgels

使用负载多粘菌素的聚（苯乙烯磺酸盐）微凝胶的自我防御抗菌表面

• DOI: 10.1021/acsbiomaterials.2c00783
• 发表时间: 2022
• 期刊: ACS Biomaterials Science & Engineering
• 影响因子: 5.8
• 作者: Xiao, Xixi;Ji, Jingjing;Wang, Haoyu;Nangia, Shikha;Wang, Hongjun;Libera, Matthew
• 通讯作者: Libera, Matthew