A nanoscale thermocouple on a micromachined cantilever for intracellular temperature measurements

微机械悬臂上的纳米级热电偶用于细胞内温度测量

• 批准号: 2226930
• 负责人: Angelo Gaitas
• 金额: $ 49.06万
• 依托单位: Icahn School of Medicine at Mount Sinai
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2226930&HistoricalAwards=false
• 关键词: nanoscale; thermocouple; micromachined; cantilever; intracellular

An award is made to the Icahn School of Medicine at Mount Sinai to develop an instrument for subcellular temperature measurements that offers high spatial and temporal resolution. This enabling instrumentation will aid in addressing basic scientific questions helping researchers derive significant new knowledge. The instrumentation will be able to uniquely provide reliable temperature measurements on the surface and inside single cells with nanoscale resolution and microseconds response time. The instrumentation will be compatible with atomic force microscopes (AFMs) that offer exquisite force detection, spatial control, and ability for fluorescence imaging. The devices will be made available to outside groups. Results and designs will be disseminated thought peer reviewed publications, conferences, websites, and online video publications. Commercialization partnerships have been established to bring this instrumentation to market once it is fully developed. Educational outreach programs to expose youths from minority and economically disadvantaged backgrounds to science include an undergraduate summer internship program and a monthly high school hosting program with interactive demonstrations and discussions. Temperature is one of the most important physiological variables and is largely not studied at the cellular level due to lack of reliable tools. The measurement of thermal events and temperature gradients at the subcellular level are of great importance to the understanding of cell function, since many biophysical and enzymatic processes can be substantially accelerated by modest increases in temperature. This enabling instrumentation is transformative in that new knowledge will be generated by measuring temperature along with other parameters at subcellular levels. The research made possible by this new instrumentation will shed light on how localized and transient changes in temperature, evoked by physiological events as disparate as cell division and synaptic stimulation, can influence the downstream effects of those events. Better understanding of these temperature-dependent biophysical processes could lead to better understanding of normal function at the cellular and tissue levels, and diseases states. Findings and comparative studies powered by this new instrument may lead to new treatments and enable the development of targeted specialized temperature-sensitive drug delivery systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

向西奈山的伊坎医学院颁发了奖励，以开发一种可提供高空间和时间分辨率的亚细胞温度测量的工具。这种启用仪器将有助于解决基本的科学问题，以帮助研究人员获得重要的新知识。该仪器将能够以纳米级分辨率和微秒响应时间在表面和单个细胞内提供可靠的温度测量值。该仪器将与原子力显微镜（AFMS）兼容，这些原子力显微镜（AFMS）提供了精美的力检测，空间控制和荧光成像的能力。设备将提供给外部组。结果和设计将被传播，经过审查的出版物，会议，网站和在线视频出版物。建立了商业化合作伙伴关系，以使该仪器充分开发到市场。教育外展计划，使年轻人从少数派和经济弱势背景的科学背景中揭露，包括一项本科暑期实习计划和每月一次的高中托管计划，并进行互动示威和讨论。温度是最重要的生理变量之一，由于缺乏可靠的工具，在细胞水平上很大程度上没有研究。亚细胞水平的热事件和温度梯度的测量对于对细胞功能的理解至关重要，因为许多生物物理和酶促过程可以通过适度的温度升高来实质上加速。这种启用仪器具有变革性，因为新知识将通过测量温度以及亚细胞水平的其他参数产生。这项新仪器使这项研究成为可能，将阐明温度的局部变化和瞬态变化，这是由像细胞分裂和突触刺激一样不同的生理事件引起的，会影响这些事件的下游效应。更好地了解这些依赖温度的生物物理过程可能会更好地理解细胞和组织水平的正常功能，以及疾病状态。由该新工具提供支持的发现和比较研究可能会导致新的治疗方法，并能够发展有针对性的专业温度敏感药物输送系统。该奖项反映了NSF的法定任务，并被认为是通过基金会的智力优点和更广泛的影响来评估的评估值得支持的。