A nanoscale thermocouple on a micromachined cantilever for intracellular temperature measurements

微机械悬臂上的纳米级热电偶用于细胞内温度测量

• 批准号: 2226930
• 负责人: Angelo Gaitas
• 金额: $ 49.06万
• 依托单位: Icahn School of Medicine at Mount Sinai
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2226930&HistoricalAwards=false
• 关键词: nanoscale; thermocouple; micromachined; cantilever; intracellular

An award is made to the Icahn School of Medicine at Mount Sinai to develop an instrument for subcellular temperature measurements that offers high spatial and temporal resolution. This enabling instrumentation will aid in addressing basic scientific questions helping researchers derive significant new knowledge. The instrumentation will be able to uniquely provide reliable temperature measurements on the surface and inside single cells with nanoscale resolution and microseconds response time. The instrumentation will be compatible with atomic force microscopes (AFMs) that offer exquisite force detection, spatial control, and ability for fluorescence imaging. The devices will be made available to outside groups. Results and designs will be disseminated thought peer reviewed publications, conferences, websites, and online video publications. Commercialization partnerships have been established to bring this instrumentation to market once it is fully developed. Educational outreach programs to expose youths from minority and economically disadvantaged backgrounds to science include an undergraduate summer internship program and a monthly high school hosting program with interactive demonstrations and discussions. Temperature is one of the most important physiological variables and is largely not studied at the cellular level due to lack of reliable tools. The measurement of thermal events and temperature gradients at the subcellular level are of great importance to the understanding of cell function, since many biophysical and enzymatic processes can be substantially accelerated by modest increases in temperature. This enabling instrumentation is transformative in that new knowledge will be generated by measuring temperature along with other parameters at subcellular levels. The research made possible by this new instrumentation will shed light on how localized and transient changes in temperature, evoked by physiological events as disparate as cell division and synaptic stimulation, can influence the downstream effects of those events. Better understanding of these temperature-dependent biophysical processes could lead to better understanding of normal function at the cellular and tissue levels, and diseases states. Findings and comparative studies powered by this new instrument may lead to new treatments and enable the development of targeted specialized temperature-sensitive drug delivery systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

西奈山伊坎医学院获得了一个奖项，以开发一种提供高空间和时间分辨率的亚细胞温度测量仪器。这种使能仪器将有助于解决基本的科学问题，帮助研究人员获得重要的新知识。该仪器将能够以纳米级分辨率和微秒级响应时间独特地提供单个电池表面和内部的可靠温度测量。该仪器将与原子力显微镜（AFM）兼容，提供精致的力检测，空间控制和荧光成像能力。这些设备将提供给外部团体。结果和设计将通过同行评审的出版物、会议、网站和在线视频出版物传播。已经建立了商业化伙伴关系，以便在该仪器完全开发后将其推向市场。教育推广计划，使来自少数民族和经济上处于不利地位的背景的青年接触科学，包括一个本科生暑期实习计划和一个每月一次的高中托管计划，其中包括互动演示和讨论。温度是最重要的生理变量之一，由于缺乏可靠的工具，在很大程度上没有在细胞水平上进行研究。在亚细胞水平上测量热事件和温度梯度对于理解细胞功能非常重要，因为许多生物物理和酶促过程可以通过适度的温度升高而大大加速。这种使能的仪器是变革性的，因为新的知识将通过测量温度沿着在亚细胞水平上的其他参数而产生。这种新仪器使研究成为可能，它将揭示由细胞分裂和突触刺激等不同的生理事件引起的局部和短暂的温度变化如何影响这些事件的下游效应。更好地理解这些温度依赖性生物物理过程可以导致更好地理解细胞和组织水平的正常功能以及疾病状态。由这一新仪器提供动力的发现和比较研究可能会导致新的治疗方法，并使有针对性的专业温度敏感药物输送系统的发展成为可能。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的知识价值和更广泛的影响审查标准进行评估来支持。