SBIR Phase I: Electrophysiology and fluidic delivery with a nano patch-clamp probe

SBIR 第一阶段：使用纳米膜片钳探针进行电生理学和流体输送

• 批准号: 1248339
• 负责人: Angelo Gaitas
• 金额: $ 15万
• 依托单位: Kytaro Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1248339&HistoricalAwards=false
• 关键词: SBIR; Phase; Electrophysiology; fluidic; delivery

This Small Business Innovation Research (SBIR) Phase I project aims to develop a micromachined patch-clamp probe that enables sub-cellular imaging, electrophysiology, and fluidic delivery at the nanoscale with control of applied force at pN levels. The probe can operate like a force sensing finger to sense contact & membrane rupture, no longer damaging cells. The small aperture allows for micro-injections and interrogation of ion channels. Additional information such as imaging, ion channel localization/mapping, and elastography can be obtained. The probe will provide new insights in the workings of cells. The broader impact/commercial potential of this project is to address the increasing need for tools to study the structure and function of single living cells. Patch clamps and micro-injectors are indispensible tools for studying cellular activity. However, these tools are plagued with numerous problems, such as damaging cells and lack of resolution, which recent innovations fail to address. The proposed nano-patch clamp addresses these problems and enables single cell analysis (SCA) for basic or clinical studies and drug discovery. The micro-machined patch clamp can become a versatile tool for SCA, which has direct implications in diagnostics, understanding disorders, and the discovery of new drugs. With the costs of clinical trials in the billion dollar range, SCA offers researchers an inexpensive way to quickly and harmlessly test and screen drug candidates on cells before testing on animals or humans. At the same time, new personalized treatments tested on the patient?s own cells, rather than one-size-fits-all treatments, have an excellent likelihood of effectively treating diseases in the future.

这项小型企业创新研究（SBIR）I阶段项目旨在开发微机械贴片钳探针，该探针在纳米级的纳米级供应下细胞成像，电生理学和流体递送，并控制PN水平的施加力。该探针可以像感测手指一样操作，以感知接触和膜破裂，而不再破坏细胞。小孔允许对离子通道进行显微注射和询问。可以获得其他信息，例如成像，离子通道定位/映射和弹性图。该探针将在细胞工作中提供新的见解。该项目的更广泛的影响/商业潜力是满足对研究单个活细胞结构和功能的越来越多的需求。斑块夹和微型注射器是研究细胞活性的必不可少的工具。但是，这些工具困扰着许多问题，例如破坏性细胞和缺乏解决方案，这些创新无法解决。拟议的纳米斑块夹解决了这些问题，并实现了基础或临床研究和药物发现的单细胞分析（SCA）。微型加工的斑块夹可以成为SCA的多功能工具，该工具对诊断，了解疾病和发现新药具有直接影响。随着临床试验在十亿美元范围内的成本，SCA为研究人员提供了一种廉价的方式，可以在对动物或人类进行测试之前快速无害地测试和筛选候选药物。同时，对患者自己的细胞进行了测试的新个性化治疗方法，而不是一种适合所有治疗方法，具有很高的有效治疗疾病的可能性。