Mechanisms of abiotic stress responses in tomato (Solanum lycopersicum)

番茄非生物胁迫反应机制（Solanum lycopersicum）

• 批准号: 253721201
• 负责人: Professor Dr. Jörg Kudla
• 金额: --
• 依托单位: UNESCO Biotechnology Center
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/253721201?language=en
• 关键词: Mechanisms; abiotic; stress; responses; tomato

The long-term goal of this project is to advance our understanding of the molecular mechanisms and the underlying genetic components that function in heat and salt stress tolerance in tomato. During the last funding period we have confirmed and initially characterized a generative heat tolerance trait of the Palestinian tomato cultivar P2. Moreover, we obtained evidence that this cultivar appears to exhibit also exceptional salt tolerance with respect to fertilization and fruit set. An emerging new concept during the last funding period was based on the identification of a transpiration dependent salt tolerance pathway in Arabidopsis. In this regard we identified not only a CBL-CIPK complex but also a ROP GTPase as so far unknown components of this pathway. We succeeded in identifying and assembling a homologous CBL-CIPK-RBOHF module from tomato and initiated genetic approaches to enable the study of the molecular and physiological function of these proteins. Based on our identification of ROP11 from Arabidopsis as a component of transpiration dependent salt tolerance we similarly performed the characterization of the small GTPase SlROP9 (the homolog of AtROP11) from tomato. Based on these achievements we intend to pursue two specific aims during the requested extension period:(i) We intend to further phenotypically characterize the heat tolerant generative trait of the P2 variety (midday heat tolerance and potential tolerance to elevated night temperatures) and to genetically identify the underlying genetic cause(s) for this abiotic stress trait(s). Similarly, we want to elucidate the characteristics and genetic causes of the generative salt tolerance that we observed in this variety. To this end we will combine in vitro and in vivo approaches, like pistil feeding assays, for detailed characterization of the heat and salt tolerant phenotypes of P2. Subsequently, we will use the already existing F2 populations for a large-scale phenotyping, initial RAPD based QTL mapping/verification and finally a map-based cloning using NGS approaches. (ii) Moreover, we will investigate the molecular mechanisms and physiological function of the tomato CBL-CIPK-RBOH-ROP module that we have assembled during the last funding period. Here the focus will be on the further generation and characterization of genetically modified tomato lines that allow to elucidate the function and physiological roles of these proteins. Together, these approaches should significantly enhance our understanding of the mechanisms and signaling networks that underlie heat and salt tolerance in tomato.

这个项目的长期目标是促进我们对番茄耐热和耐盐的分子机制和潜在的遗传成分的理解。在上一次资助期间，我们确认并初步确定了巴勒斯坦番茄品种P2的耐热生殖特性。此外，我们获得的证据表明，该品种在受精和座果方面似乎也表现出特殊的耐盐性。在上一次筹资期间出现的一个新概念是基于对拟南芥中蒸腾作用依赖的耐盐性途径的鉴定。在这方面，我们不仅发现了一个CBL-CIPK复合体，而且还发现了一个ROP GTP酶，这些都是迄今为止未知的该途径的组成部分。我们成功地从番茄中鉴定和组装了一个同源的CBL-CIPK-RBOHF模块，并启动了遗传方法来研究这些蛋白的分子和生理功能。在我们从拟南芥中鉴定出ROP11是蒸腾作用依赖的耐盐性成分的基础上，我们类似地对番茄中的小GTP酶SlROP9(AtROP11的同源基因)进行了鉴定。基于这些成果，我们打算在所要求的延长期内追求两个具体目标：(I)我们打算进一步表征P2品种的耐热繁殖性状(中午耐热和潜在的夜间高温耐性)，并从遗传上确定这种非生物胁迫性状的潜在遗传原因(S)(S)。同样，我们想要阐明我们在该品种中观察到的生殖耐盐性的特征和遗传原因。为此，我们将结合体外和体内的方法，如雌蕊饲养试验，详细描述P2的耐热和耐盐表型。随后，我们将利用已经存在的F2群体进行大规模的表型鉴定，初步的基于RAPD的QTL定位/验证，最后使用NGS方法进行基于图谱的克隆。(Ii)此外，我们还将研究我们在上一个资助期组装的番茄CBL-CIPK-RBOH-ROP模块的分子机制和生理功能。在这里，重点将放在进一步的转基因番茄品系的产生和特征上，从而能够阐明这些蛋白质的功能和生理作用。总之，这些方法将显著提高我们对番茄耐热性和耐盐性的机制和信号网络的理解。