Functional analysis of a calcium sensor protein/serine-threonine kinase signalling network

钙传感器蛋白/丝氨酸-苏氨酸激酶信号网络的功能分析

• 批准号: 5352716
• 负责人: Professor Dr. Jörg Kudla
• 金额: --
• 依托单位: Arbeitsgruppe Molecular Genetics and Cell Biology of Plants
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5352716?language=en
• 关键词: Functional; analysis; calcium; sensor; protein

Calcium signals regulate a multitude of biological responses and cellular processes in eukaryotic organisms. Although considerable progress has been made in elucidating physiological processes involving calcium signals in plants, the identity of most of the components involved and the mechanisms generating specificity in this signalling cascades are only beginning to be understood. This project aims to comprehensively explore the mechanisms of a novel calcium signalling system from Arabidopsis. Based on the recent identification of a specific group of serine-threonine protein kinases (AtCIPKs) as interacting targets of the Arabidopsis calcineurin B-like calcium sensor proteins (AtCBLs) this project is focused on the functional characterisation of the CBL-CIPK protein-protein interaction network and its contribution to integrate and specifically decode the various calcium signals in plant cells. The proposed research addresses the folowing main objectives: (1) A thorough molecular characterisation of the CBL and CIPK protein families by their expression, subcelluar localisation, biochemical properties and interaction specificy. (2) The identification of the specific physiological processes by the diffrential calcium sensor/kinase complex formation. This approach will rely on the isolation and detailed phenotypical physiological and genetic charakterisation of mutant lines generated by reserve genetic approaches as well as on interaction analyses by yeast two-hybrid assays. (3) The identification and analyses of proteins regulated by the CBL-CIPK complexes by means of large scale two- and three-hybrid screens.

钙信号调节真核生物的多种生物反应和细胞过程。虽然在阐明植物中涉及钙信号的生理过程方面已经取得了相当大的进展，但所涉及的大多数组分的身份和在此信号级联中产生特异性的机制才刚刚开始被理解。本项目旨在从拟南芥中全面探索一种新的钙信号系统的机制。基于最近发现的一组丝氨酸-苏氨酸蛋白激酶（AtCIPKs）作为拟南芥钙调神经磷酸酶B样钙传感蛋白（AtCBLs）的相互作用靶点，本项目重点研究CBL-CIPK蛋白-蛋白相互作用网络的功能特征及其在整合和特异性解码植物细胞中各种钙信号中的作用。本研究的主要目的是：（1）从CBL和CIPK蛋白家族的表达、亚细胞定位、生化特性和相互作用特异性等方面对CBL和CIPK蛋白家族进行全面的分子表征。(2)通过钙离子传感器/激酶复合物的形成识别特定的生理过程。这种方法将依赖于隔离和详细的表型生理和遗传charakterisation的突变株系所产生的储备遗传方法，以及酵母双杂交试验的相互作用分析。(3)通过大规模双杂交和三杂交筛选，鉴定和分析CBL-CIPK复合物调控的蛋白质。