Function and calcium dependent regulation of CBL/CIPK-mediated protein phosphorylation

该项目旨在研究由钙调磷酸酶B样蛋白（CBL）及其相互作用蛋白激酶（CIPKs）组成的复杂信号网络对植物钙信号过程的贡献。总的来说，该项目的重点是蛋白质磷酸化过程作为产生和解码钙信号的重要步骤。具体而言，该项目旨在：（i）研究作为调节CBL/CIPK复合物活性的功能性原理的蛋白质磷酸化，（ii）研究CBL/CIPK在调节ABA应答性转录因子中的作用，（iii）研究CBL/CIPK与PP 2C在调节ABA感知中的相互作用，（iv）研究CBL/CIPK与CDPK和SnRK 2在调节离子转运中的相互作用，（v）通过五重cbl突变体的比较磷酸蛋白质组学分析，通过磷酸化底物的无偏倚鉴定来鉴定和表征CBL/CIPK靶标，和（vi）以高组织特异性和亚细胞分辨率分析响应于阿坝或冷的Ca 2+浓度的动力学。

The proposed project aims to investigate the contribution of a complex signaling network comprising Calcineurin B-like proteins (CBLs) and their interacting protein kinases (CIPKs) to calcium signaling processes in plants. In general, this project is focused on protein phosphorylation processes as important steps in generating and decoding calcium signals. Specifically, this project aims to: (i) investigate protein phosphorylation as functional principle regulating CBL/CIPK complex activity, (ii) investigate the role of CBL/CIPKs in regulating ABA-responsive transcription factors, (iii) investigate the interconnection of CBL/CIPKs with PP2Cs in regulating ABAperception, (iv) investigate the interplay of CBL/CIPKs with CDPKs and SnRK2s in regulating ion transport, (v) identify and characterize CBL/CIPK targets by the nonbiased identification of phosphorylation substrates by comparative phosphoproteomics analyses of quintuple cbl mutants, and (vi) analyze the dynamics of Ca2+ concentration in response to ABA or cold at high tissue-specific and sub-cellular resolution.