Central imaging facility for advanced light and correlated light and electron microscopy (Z01)

Z01项目为最先进的技术和资源提供了一个中心平台，如1）超分辨率受激发射耗尽（STED）和双光子共聚焦显微镜，2）智能自动化延时成像，3）高压冷冻（HPF）样品制备，4）相关光学和电子显微镜（CLEM）和5）3D EM重建。该平台将可用于CRC 1140的所有项目组。我们将专注于开发新的自动化成像方法，用于在线跟踪3D生物结构，以及用于3D EM重建的肾组织结构保留嵌入方法等。一个全面的课程和标准操作说明将确保设备和方法的有效使用。

The project Z01 provides a central platform for state-of-the-art technologies and resources like 1) super-resolution stimulated emission depletion (STED) and two-photon confocal microscopy, 2) intelligent, automatized time-lapse imaging, 3) high-pressure freeze (HPF) sample preparation, 4) correlated light and electron microscopy (CLEM) and 5) 3D EM reconstructions. This platform will be available for all project groups of the CRC 1140. We will focus on the development of new automatized imaging methods for online tracking of biological structures in 3D as well as methods for structure-preserving embedding of kidney tissues for 3D EM reconstructions etc. A comprehensive program with courses and standard operating instructions will ensure an effective use of the equipment and methods.