From discrete particle to single cell catalysis

从离散颗粒到单细胞催化

• 批准号: 275564491
• 负责人: Professor Dr. Detlev Belder
• 金额: --
• 依托单位: Dipartimento di Scienze Chimiche, Farmaceutiche ed Agrarie (DOCPAS)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/275564491?language=en
• 关键词: discrete; particle; single; cell; catalysis

Microfluidic systems enable the analysis of processes on the single particle or cell level. While single cell studies based on fluorescence detection are the current state of the art in the life sciences and biochemical research, respective activities in synthetic chemistry and biocatalysis are still missing. The goal of project 1 is to develop enabling technologies, which allow the study of heterogeneously catalyzed organic syntheses as well as whole cell catalysis at the smallest possible dimensions. The ultimate goal is to explore stereoselective conversions in solution down to the single catalyst particle level. This challenge will be tackled by developing approaches to encapsulate, incubate, and analyse single particles in microfluidic droplet chips. These devices are then seamlessly hyphenated to analysis techniques such as mass spectrometry, ion mobility spectrometry, and chip-based liquid chromatography to monitor the turn-over and the selectivity of single catalyst species. Eventually this approach could shed light on the exciting question if and how individual catalyst species differ in enantioselectivity, which in turn would contribute to the basic understanding and the development of heterogeneous as well as whole cell catalysis.

微流体系统能够在单个颗粒或细胞水平上分析过程。虽然基于荧光检测的单细胞研究是生命科学和生物化学研究中的当前最先进技术，但合成化学和生物催化中的相应活动仍然缺失。项目1的目标是开发使能技术，允许在尽可能小的尺寸下研究多相催化有机合成以及全细胞催化。最终的目标是探索在溶液中的立体选择性转化下降到单个催化剂颗粒的水平。这一挑战将通过开发在微流控液滴芯片中封装、孵育和分析单个颗粒的方法来解决。然后，这些设备与质谱法、离子迁移谱法和基于芯片的液相色谱法等分析技术无缝连接，以监测单个催化剂物种的转化率和选择性。最终，这种方法可以阐明令人兴奋的问题，如果以及如何个别催化剂物种不同的对映体选择性，这反过来又将有助于基本的理解和发展的多相以及全细胞催化。