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Digital microfluidics (DMF) for SERS analysis on a chip with EWOD-/(w)OEW-actuated analyte droplets

使用 EWOD/(w)OEW 驱动的分析物液滴在芯片上进行 SERS 分析的数字微流体 (DMF)

基本信息

批准号：
366065598
负责人：
Professor Dr. Detlev Belder
金额：
--
依托单位：
Institut für Analytische Chemie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2017
资助国家：
德国
起止时间：
2016-12-31 至 2021-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/366065598?language=en
关键词：
Digital microfluidics DMF SERS analysis

项目摘要

Electrically conducting fluid droplets can be actuated via electrowetting on dielectric (EWOD) and opto-electrowetting (OEW). In principle, the OEW control lightwave might even be waveguided within the layer sequence beneath the droplet (idea Fouckhardt, waveguide-based OEW = wOEW). An important application is represented by droplet manipulation in digital microfluidics (DMF, lab on a chip 2.0) in chemical chip-sized laboratories.An obstacle for a break-through of digital microfluidics in miniaturized chemistry is given by a lack of sensitive, non-destructive methods for detection, allowing for the doubtless identification of chemical substances in discrete droplets on a DMF chip. In the project proposed here surface-enhanced Raman scattering or spectroscopy (SERS) shall be employed for this purpose on a chip for the first time (idea Belder), which shall make well evaluable vibrational spectra possible. By the interaction of the analyte molecules (within the droplets) e. g. with metallic nanoparticles dispersed in the droplets high signal amplifications should be obtainable.The undertaking is aimed at the first-time combination of DMF and SERS and the concurrent droplet actuation by EWOD-OEW-wOEW. By the optical initiation of the droplet movement new opportunities for the realization of unpretentious lab on a chip systems should arise.The cooperation of the groups Belder and Fouckhardt should provide for synergies, which make a break-through of the DMF technology in the context of chemical lab-chips possible. The Belder group focuses on SERS detection within DMF systems. The Fouckhardt group concentrates on further development of droplet actuation via EWOD and OEW, preferably up to wOEW.Moreover, in close cooperation the potential of solid metallic SERS targets is to be investigated, which might simultaneously be used as electrical contacts or mirror layers for the EWOD-(w)OEW technologies. Multifunctionality of components is to be considered as inherently positive for further miniaturization of DMF lab chips.In principle, for any of the SERS target alternatives any of the mentioned actuation variants, i. e. EWOD, OEW, or wOEW, might be used. The (preliminary) allocation in the work program is owed to the temporal coordination of work packages of both groups.In case of need, for droplet actuation OEW (compared with wOEW) or even EWOD (compared with wOEW and OEW) might act as a fall-back position. Even the combination of DMF and SERS and EWOD actuation would be new.

导电流体液滴可以通过在介质上的电润湿(EWOD)和光电润湿(OEW)来驱动。原则上，光波控制甚至可能在液滴下面的层序列内进行波导(Idea Fuchardt，基于波导的光波=Woew)。在化学芯片规模的实验室中，数字微流控(DMF，Lab On a Chip 2.0)中的液滴操纵是一个重要的应用。由于缺乏灵敏、无损的检测方法，无法在DMF芯片上识别离散液滴中的化学物质，这是数字微流控在微型化化学中取得突破的障碍。在这里提出的项目中，表面增强拉曼散射或光谱(SERS)将首次在芯片上用于这一目的(IDEA BELDER)，这将使很好地评估振动光谱成为可能。通过分析物分子(在液滴中)的相互作用，例如与分散在液滴中的金属纳米颗粒的相互作用，应该可以获得高信号放大。这项工作的目标是首次将DMF和SERS结合起来，并由EWOD-OEW-Woew同时驱动液滴。通过液滴运动的光学启动，应该为实现朴实无华的芯片上实验室系统提供新的机会。Belder和Fuchardt小组的合作应该提供协同效应，这使得DMF技术在化学实验室芯片方面的突破成为可能。Belder小组专注于DMF系统内的SERS检测。福克哈特小组专注于通过EWOD和OW进一步开发液滴驱动，最好是WOW。此外，还将密切合作，研究固体金属SERS靶材的潜力，这些靶材可能同时用作EWOD-(W)OW技术的电触点或镜面层。组件的多功能性被认为是DMF实验室芯片进一步小型化的内在积极因素。原则上，对于任何SERS目标替代方案，可以使用所提到的任何驱动变体，即EWOD、OEW或WOEW。工作计划中的(初步)分配是由于两组工作包在时间上的协调。如果需要，液滴驱动OWW(与Woew相比)，甚至EWOD(与Woew和OEW相比)可以作为后备位置。即使是DMF和SERS以及EWOD驱动的组合也将是新的。