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Digital microfluidics (DMF) for SERS analysis on a chip with EWOD-/(w)OEW-actuated analyte droplets

使用 EWOD/(w)OEW 驱动的分析物液滴在芯片上进行 SERS 分析的数字微流体 (DMF)

基本信息

批准号：
366065598
负责人：
Professor Dr. Detlev Belder
金额：
--
依托单位：
Institut für Analytische Chemie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2017
资助国家：
德国
起止时间：
2016-12-31 至 2021-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/366065598?language=en
关键词：
Digital microfluidics DMF SERS analysis

项目摘要

Electrically conducting fluid droplets can be actuated via electrowetting on dielectric (EWOD) and opto-electrowetting (OEW). In principle, the OEW control lightwave might even be waveguided within the layer sequence beneath the droplet (idea Fouckhardt, waveguide-based OEW = wOEW). An important application is represented by droplet manipulation in digital microfluidics (DMF, lab on a chip 2.0) in chemical chip-sized laboratories.An obstacle for a break-through of digital microfluidics in miniaturized chemistry is given by a lack of sensitive, non-destructive methods for detection, allowing for the doubtless identification of chemical substances in discrete droplets on a DMF chip. In the project proposed here surface-enhanced Raman scattering or spectroscopy (SERS) shall be employed for this purpose on a chip for the first time (idea Belder), which shall make well evaluable vibrational spectra possible. By the interaction of the analyte molecules (within the droplets) e. g. with metallic nanoparticles dispersed in the droplets high signal amplifications should be obtainable.The undertaking is aimed at the first-time combination of DMF and SERS and the concurrent droplet actuation by EWOD-OEW-wOEW. By the optical initiation of the droplet movement new opportunities for the realization of unpretentious lab on a chip systems should arise.The cooperation of the groups Belder and Fouckhardt should provide for synergies, which make a break-through of the DMF technology in the context of chemical lab-chips possible. The Belder group focuses on SERS detection within DMF systems. The Fouckhardt group concentrates on further development of droplet actuation via EWOD and OEW, preferably up to wOEW.Moreover, in close cooperation the potential of solid metallic SERS targets is to be investigated, which might simultaneously be used as electrical contacts or mirror layers for the EWOD-(w)OEW technologies. Multifunctionality of components is to be considered as inherently positive for further miniaturization of DMF lab chips.In principle, for any of the SERS target alternatives any of the mentioned actuation variants, i. e. EWOD, OEW, or wOEW, might be used. The (preliminary) allocation in the work program is owed to the temporal coordination of work packages of both groups.In case of need, for droplet actuation OEW (compared with wOEW) or even EWOD (compared with wOEW and OEW) might act as a fall-back position. Even the combination of DMF and SERS and EWOD actuation would be new.

导电流体液滴可以经由电介质上电润湿（EWOD）和光电润湿（OEW）来致动。原则上，OEW控制光波甚至可以在液滴下方的层序列内被波导（想法Fouckhartt，基于波导的OEW = wOEW）。一个重要的应用是在化学芯片大小的实验室中的数字微流体（DMF，芯片上的实验室2.0）中的液滴操作。数字微流体在小型化化学中的突破的障碍是缺乏灵敏的、非破坏性的检测方法，这使得可以在DMF芯片上毫无疑问地识别离散液滴中的化学物质。在这里提出的项目中，表面增强拉曼散射或光谱学（Sers）将首次用于芯片上的此目的（Belder的想法），这将使良好的可评估的振动光谱成为可能。通过分析物分子（在液滴内）的相互作用，G.该项目旨在首次将DMF和Sers结合起来，并通过EWOD-OEW-wOEW实现液滴的同时驱动。通过液滴运动的光学启动，实现简单的芯片实验室系统的新机会应该出现。Belder和Fouckhardt团队的合作应该提供协同作用，这使得DMF技术在化学实验室芯片背景下的突破成为可能。Belder集团专注于DMF系统中的Sers检测。Fouckhardt小组致力于通过EWOD和OEW（最好是wOEW）进一步开发液滴驱动。此外，将密切合作研究固体金属Sers靶的潜力，这些靶可能同时用作EWOD-（w）OEW技术的电接触或镜面层。元件的多功能性被认为是DMF实验室芯片进一步小型化的内在积极因素。e.可能会使用EWOD、OEW或wOEW。工作计划中的（初步）分配是由于两个小组的工作包的时间协调。在需要的情况下，对于液滴启动，OEW（与wOEW相比）或甚至EWOD（与wOEW和OEW相比）可以作为后备位置。甚至DMF和Sers和EWOD驱动的组合也将是新的。