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Digital microfluidics (DMF) for SERS analysis on a chip with EWOD-/(w)OEW-actuated analyte droplets

使用 EWOD/(w)OEW 驱动的分析物液滴在芯片上进行 SERS 分析的数字微流体 (DMF)

基本信息

批准号：
366065598
负责人：
Professor Dr. Detlev Belder
金额：
--
依托单位：
Institut für Analytische Chemie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2017
资助国家：
德国
起止时间：
2016-12-31 至 2021-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/366065598?language=en
关键词：
Digital microfluidics DMF SERS analysis

项目摘要

Electrically conducting fluid droplets can be actuated via electrowetting on dielectric (EWOD) and opto-electrowetting (OEW). In principle, the OEW control lightwave might even be waveguided within the layer sequence beneath the droplet (idea Fouckhardt, waveguide-based OEW = wOEW). An important application is represented by droplet manipulation in digital microfluidics (DMF, lab on a chip 2.0) in chemical chip-sized laboratories.An obstacle for a break-through of digital microfluidics in miniaturized chemistry is given by a lack of sensitive, non-destructive methods for detection, allowing for the doubtless identification of chemical substances in discrete droplets on a DMF chip. In the project proposed here surface-enhanced Raman scattering or spectroscopy (SERS) shall be employed for this purpose on a chip for the first time (idea Belder), which shall make well evaluable vibrational spectra possible. By the interaction of the analyte molecules (within the droplets) e. g. with metallic nanoparticles dispersed in the droplets high signal amplifications should be obtainable.The undertaking is aimed at the first-time combination of DMF and SERS and the concurrent droplet actuation by EWOD-OEW-wOEW. By the optical initiation of the droplet movement new opportunities for the realization of unpretentious lab on a chip systems should arise.The cooperation of the groups Belder and Fouckhardt should provide for synergies, which make a break-through of the DMF technology in the context of chemical lab-chips possible. The Belder group focuses on SERS detection within DMF systems. The Fouckhardt group concentrates on further development of droplet actuation via EWOD and OEW, preferably up to wOEW.Moreover, in close cooperation the potential of solid metallic SERS targets is to be investigated, which might simultaneously be used as electrical contacts or mirror layers for the EWOD-(w)OEW technologies. Multifunctionality of components is to be considered as inherently positive for further miniaturization of DMF lab chips.In principle, for any of the SERS target alternatives any of the mentioned actuation variants, i. e. EWOD, OEW, or wOEW, might be used. The (preliminary) allocation in the work program is owed to the temporal coordination of work packages of both groups.In case of need, for droplet actuation OEW (compared with wOEW) or even EWOD (compared with wOEW and OEW) might act as a fall-back position. Even the combination of DMF and SERS and EWOD actuation would be new.

导电液滴可以通过电介质电润湿 (EWOD) 和光电润湿 (OEW) 来驱动。原则上，OEW 控制光波甚至可以在液滴下方的层序列内进行波导（想法 Fouckhardt，基于波导的 OEW = wOEW）。一个重要的应用是化学芯片大小的实验室中数字微流控（DMF，芯片实验室2.0）中的液滴操纵。数字微流控在小型化化学领域取得突破的一个障碍是缺乏灵敏、非破坏性的检测方法，从而可以毫无疑问地识别DMF芯片上离散液滴中的化学物质。在此提出的项目中，表面增强拉曼散射或光谱学（SERS）将首次在芯片上用于此目的（贝尔德的想法），这将使可良好评估的振动光谱成为可能。通过分析物分子（液滴内）的相互作用 e． g。当金属纳米颗粒分散在液滴中时，应该可以获得高信号放大。该项目的目的是首次将DMF和SERS结合起来，并通过EWOD-OEW-wOEW同时进行液滴驱动。通过液滴运动的光学启动，应该出现实现朴素的芯片实验室系统的新机会。Belder 和 Fouckhardt 团队的合作应该提供协同效应，从而使 DMF 技术在化学实验室芯片方面的突破成为可能。 Belder 小组专注于 DMF 系统内的 SERS 检测。 Fouckhardt 小组专注于通过 EWOD 和 OEW 进一步开发液滴驱动，最好达到 wOEW。此外，通过密切合作，将研究固体金属 SERS 目标的潜力，该目标可能同时用作 EWOD-(w)OEW 技术的电接触或镜面层。组件的多功能性被认为对于 DMF 实验室芯片的进一步小型化具有内在的积极作用。原则上，对于任何 SERS 目标替代方案，任何提到的驱动变体，即。 e.可以使用 EWOD、OEW 或 wOEW。工作计划中的（初步）分配归功于两个小组工作包的时间协调。在需要的情况下，对于液滴驱动OEW（与wOEW相比）甚至EWOD（与wOEW和OEW相比）可以作为后备位置。甚至 DMF 和 SERS 以及 EWOD 驱动的组合也是新的。