Unraveling solitary bee microbiota: ecology, importance during development and function

解开独居蜜蜂微生物群：生态学、发育和功能过程中的重要性

• 批准号: 279735434
• 负责人: Professor Dr. Alexander Keller
• 金额: --
• 依托单位: Professur für Zelluläre und Organismische Netzwerke
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/279735434?language=en
• 关键词: Unraveling; solitary; bee; microbiota; ecology

Bees are considered to be very important agents for ecological networks and agricultural systems, mostly through their incommensurable pollination services. As recent studies demonstrated, it is however not only the abundance of bees that regulates ecosystem benefits, but also a diversity of pollinators is required to maintain effective pollination. As solitary bees contribute largely to overall pollination of natural and cultivated plants as diverse and effective pollinators, it is of high interest and goal of this study to gain knowledge about their effectiveness in recruiting offspringWe aim to identify natural microbiota of several bee species from different landscapes, relating it to ecological factors of importance and their origin in different nesting materials. This will be supplemented with a comprehensive pathological investigation of microbial agents found in diseased and dead solitary bee larvae. Further, we will apply experimental tests with bacterial suppression through antibiotic substances. We aim to identify changes in bee survivability and investigate the microbiota at different developmental stages of the larvae within a standardized setup. Lastly, we will analyse in detail the importance of bacteria in fermentation processes and antifungal activity. This is done by bioassays that monitor fungal growth/repression as well as changes in the pollen constitution and released nutrients in presence of bacteria isolated and cultivated from brood chambers. Further, we will investigate through shotgun sequencing the genomic properties of bacteria that show positive assays. These will be compared to those of known fermentation capabilities, i.e. lactic acid bacteria, and antifungal activity in Hymenoptera, e.g. Streptomyces, and studied for shared genes and pathways.The combined interpretation of these results will largely contribute to our general understanding of solitary bee development in relation to microbiotic interactions.

蜜蜂被认为是生态网络和农业系统中非常重要的代理人，主要是通过它们不可比拟的授粉服务。然而，正如最近的研究表明的那样，不仅蜜蜂的丰富程度调节了生态系统的利益，而且还需要传粉媒介的多样性来维持有效的授粉。由于独居蜜蜂作为一种多样化和有效的传粉媒介，在自然和人工植物的授粉中起着重要的作用，因此了解它们在招募后代方面的有效性是本研究的高度兴趣和目标。我们的目标是鉴定不同景观中几种蜜蜂的天然微生物群，将其与重要的生态因素及其在不同筑巢材料中的来源联系起来。这将与在患病和死亡的独居蜜蜂幼虫中发现的微生物剂的全面病理调查相补充。此外，我们将通过抗生素物质进行细菌抑制的实验测试。我们的目标是确定蜜蜂生存能力的变化，并在标准化设置中调查幼虫不同发育阶段的微生物群。最后，我们将详细分析细菌在发酵过程中的重要性和抗真菌活性。这是通过监测真菌生长/抑制以及花粉构成的变化和从育卵室分离培养的细菌存在下释放的营养物质的生物测定来完成的。此外，我们将通过鸟枪测序研究显示阳性的细菌的基因组特性。这些将与已知的发酵能力（如乳酸菌）和膜翅目（如链霉菌）的抗真菌活性进行比较，并研究共享基因和途径。对这些结果的综合解释将在很大程度上有助于我们对与微生物相互作用有关的独居蜜蜂发育的一般理解。

项目成果

Wild bees and their nests host Paenibacillus bacteria with functional potential of avail

• DOI: 10.1186/s40168-018-0614-1
• 发表时间: 2018-12
• 期刊: Microbiome
• 影响因子: 15.5
• 作者: A. Keller;Annette Brandel;Mira C. Becker;Rebecca Balles;U. Abdelmohsen;M. Ankenbrand;Wiebke Sickel

Linking pollen foraging of megachilid bees to their nest bacterial microbiota

将巨幼蜂的花粉觅食与其巢细菌微生物群联系起来

• DOI: 10.1002/ece3.5599
• 发表时间: 2019
• 期刊: Ecology and Evolution
• 影响因子: 2.6
• 作者: Voulgari-Kokota A;Ankenbrand M;Grimmer G;Steffan-Dewenter I;Keller A.
• 通讯作者: Keller A.

Drivers, Diversity, and Functions of the Solitary-Bee Microbiota.

• DOI: 10.1016/j.tim.2019.07.011
• 发表时间: 2019-12
• 期刊: Trends in microbiology
• 影响因子: 15.9
• 作者: A. Voulgari-Kokota;Q. McFrederick;I. Steffan‐Dewenter;A. Keller

Bacterial community structure and succession in nests of two megachilid bee genera

• DOI: 10.1093/femsec/fiy218
• 发表时间: 2018-10
• 期刊: FEMS Microbiology Ecology
• 影响因子: 4.2
• 作者: A. Voulgari-Kokota;G. Grimmer;I. Steffan‐Dewenter;A. Keller