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The evolutionary origin of jasmonate signalling and biosynthesis

茉莉酸信号传导和生物合成的进化起源

基本信息

批准号：
285640135
负责人：
Professorin Dr. Christiane Gatz
金额：
--
依托单位：
Abteilung Molekularbiologie und Physiologie der Pflanze
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2015
资助国家：
德国
起止时间：
2014-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/285640135?language=en
关键词：
evolutionary origin jasmonate signalling biosynthesis

项目摘要

Higher plants respond to mechanical wounding and herbivory with the synthesis of the plant hormone jasmonoyl-isoleucine (JA-Ile) which in turn induces defense responses, both in local and systemic leaves. The model organism Arabidopsis thaliana has been instrumental in elucidating the JA-Ile-activated signal transduction chain, which starts with the formation of a JA-Ile-induced interaction between the F-Box protein COI1 and members of the family of JAZ repressors. COI1-mediated degradation of JAZ repressors is the key step for the activation of transcription. In this proposal, we are interested in the evolution of JA-Ile synthesis and its cognate COI1/JAZ-dependent perception. Starting point of the project is the finding that the liverwort Marchantia polymorpha genome/transcriptome contains MpCOI1 sequences, which show high similarity to AtCOI1. Amino acids which contact the ligand and/or the JAZ repressor degron are highly conserved. Moreover, a single JAZ gene encoding a degron peptide with almost the same amino acid sequence as the degron peptide of JAZs from angiosperms is present in the Marchantia genome. However, no JA or JA-Ile is detected in wounded thalli of M. polymorpha, which is consistent with the absence of the JA-Ile biosynthesis enzyme OPDA Reductase 3 (OPR3) in the publicly available genome and transcriptome sequences. In this proposal we aim to answer the following questions: (1) When during the evolution of land plants did JA biosynthesis and perception of JA-Ile by the COI1/JAZ co-receptor complex emerge? (2) What are the functions of COI1 and JAZ in M. polymorpha? (3) Is there a ligand that is perceived by the COI1/JAZ module in M. polymorpha?

高等植物对机械损伤和植食性的反应是合成植物激素茉莉酰-异亮氨酸（JA-Ile），其反过来诱导局部和系统叶片的防御反应。模式生物拟南芥一直有助于阐明JA-Ile激活的信号转导链，该信号转导链始于F-Box蛋白COI 1和JAZ阻遏物家族成员之间JA-Ile诱导的相互作用的形成。COI 1介导的JAZ阻遏物降解是转录激活的关键步骤。在这个提议中，我们感兴趣的是JA-Ile合成及其同源COI 1/JAZ依赖性感知的演变。该项目的出发点是发现地钱基因组/转录组包含MpCOI 1序列，其与AtCOI 1具有高度相似性。与配体和/或JAZ阻遏物降解决定子接触的氨基酸是高度保守的。此外，地钱属基因组中存在编码降解决定子肽的单个JAZ基因，该降解决定子肽具有与来自被子植物的JAZ的降解决定子肽几乎相同的氨基酸序列。而在M.多形藻中，这与在公众可获得的基因组和转录组序列中不存在JA-Ile生物合成酶OPDA还原酶3（OPR 3）一致。在这个提议中，我们的目标是回答以下问题：（1）在陆地植物的进化过程中，JA的生物合成和JA-Ile的COI 1/JAZ共受体复合物的感知出现？(2)COI 1和JAZ在M. polymorpha？(3)是否存在被M中的COI 1/JAZ模块感知的配体。polymorpha？