Identification of direct target genes of the Arabidopsis DELLA proteins RGA and GAI using the ChIP-chip technology

使用 ChIP 芯片技术鉴定拟南芥 DELLA 蛋白 RGA 和 GAI 的直接靶基因

• 批准号: 50069580
• 负责人: Professorin Dr. Christiane Gatz
• 金额: --
• 依托单位: Lehrstuhl für Systembiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/50069580?language=en
• 关键词: Identification; direct; target; genes; Arabidopsis

The phytohormone gibberellic acid (GA) is involved in the regulation of important aspects of plant growth and development including germination, stem elongation and the control of flowering time. GA responses require the GA-dependent degradation of the DELLA repressor proteins. DELLA proteins (RGA and GAI in Arabidopsis) belong to the group of GRAS proteins. GRAS proteins have been suggested to act as transcriptional regulators, though direct binding to specific DNA sequences has not been reported yet. Using chromatin immunoprecipitation experiments (ChIP), we have recently demonstrated that the GRAS protein SCARECROWLIKE-14 is recruited to specific DNA sequences by DNA-bound bZIP transcription factors of the TGA family. Having established a reliable ChIP protocol for GRAS proteins we propose to identify target promoters for GAI and RGA using ChIP analysis in combination with the hybridization of ChIP enriched sequences to a tiling promoter array (ChIP-chip). The project has the potential to close an important gap in understanding GA signalling in plants.

植物激素赤霉酸（GA）参与调节植物生长和发育的重要方面，包括发芽、茎伸长和开花时间的控制。GA反应需要DELLA阻遏蛋白的GA依赖性降解。DELLA蛋白（拟南芥中的RGA和GAI）属于GRAS蛋白。GRAS蛋白被认为是转录调节因子，但与特定DNA序列的直接结合还没有报道。使用染色质免疫沉淀实验（ChIP），我们最近已经证明，GRAS蛋白SCARECROWLIKE-14被招募到特定的DNA序列的TGA家族的DNA结合bZIP转录因子。已经建立了一个可靠的ChIP协议的GRAS蛋白，我们建议确定目标启动子GAI和RGA使用ChIP分析结合杂交的ChIP富集序列的平铺启动子阵列（ChIP芯片）。该项目有可能缩小理解植物GA信号传导的重要差距。