Mechanistically Novel Group of Membrane Transporters

机制新颖的膜转运蛋白组

• 批准号: 30656198
• 负责人: Professor Dr. Thomas Eitinger
• 金额: --
• 依托单位: Arbeitsgruppe Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/30656198?language=en
• 关键词: Mechanistically; Novel; Group; Membrane; Transporters

ATP-binding cassette-containing (ABC) transport systems form a huge group of membrane transporters in all kingdoms of life in which they play diverse roles such as nutrient uptake, protein translocation, ion homeostasis, and drug resistance. A special feature of all previously known uptake ABC transporters in prokaryotes is the existence of extracellular substrate-binding proteins. These components act as solute receptors and as switches that stimulate ATP hydrolysis and substrate translocation through signalling substrate availability to the cytoplasmic ABC domains. In preliminary work, we have identified several hundred members of a novel group of ABC uptake systems for metals and organic solutes, the mechanism of which differs from the general concept in several respects. These transporters contain ABC modules and are driven by ATP hydrolysis. On the other hand, they display a basic activity in the absence of an ABC module and both activities are independent of extracellular solute-binding proteins. We are convinced that a detailed biochemical characterization of these systems will uncover features that are relevant in general for an understanding of the mechanism of ABC-type transport systems.

atp结合盒-含（ABC）转运系统构成了一个庞大的膜转运体群，在所有生命领域中发挥着不同的作用，如营养摄取、蛋白质转运、离子稳态和耐药性。在原核生物中，所有已知的摄取ABC转运体的一个特殊特征是细胞外底物结合蛋白的存在。这些组分作为溶质受体和开关，通过向细胞质ABC结构域发送底物可用性信号，刺激ATP水解和底物易位。在初步工作中，我们已经确定了数百个新型金属和有机溶质ABC吸收系统的成员，其机制在几个方面与一般概念不同。这些转运体包含ABC模块，由ATP水解驱动。另一方面，它们在没有ABC模块的情况下表现出基本的活性，并且这两种活性都独立于细胞外溶质结合蛋白。我们相信，对这些系统进行详细的生化表征将揭示与理解abc型转运系统机制相关的一般特征。