Impact of gene suppression and food processing on the IgE-reactivity and allergenicity of plant derived foods

基因抑制和食品加工对植物源性食品 IgE 反应性和过敏原性的影响

• 批准号: 30717861
• 负责人: Professor Dr. Thomas Henle
• 金额: --
• 依托单位: Paul-Ehrlich-Institut - Bundesinstitut für Impfstoffe und biomedizinische Arzneimittel
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/30717861?language=en
• 关键词: Impact; gene; suppression; food; processing

Major objectives of this sub-project are to select tomato allergens as targets for the design of hypoallergenic tomato fruits by simultaneous silencing, to asses the IgE-inducing capacity in mice and allergenic potency of transgenic tomato and carrot plants, and to investigate the impact of protein glycation on allergenicity. Allergenic potency will be assessed by IgE-binding and mediator release assays with transgenic plants, with or without exposure to biotic and abiotic stress conditions. Since several target proteins are involved in plant defence the sub-project aims at investigating the potential up regulation of compensatory allergenic pathogen-related proteins. In cases in which essential cellular functions are targeted by the RNAi approach, homologous proteins (i.e. profilin) with reduced allergenic potency will be selected or generated to substitute the biological function. Furthermore, the impact of modifications occurring during food processing on allergenic potency, T-cell reactivity, and IgE inducing capacity in mice will be investigated by testing selected allergens and allergen peptides subjected to glycation reactions. Moreover, individual glycation compounds will be synthesized for subsequent covalent coupling to selected target proteins and products analysed using the same methodology.

这个子项目的主要目标是选择番茄过敏原作为设计同时沉默低过敏番茄水果的靶标，评估转基因番茄和胡萝卜植株在小鼠体内诱导IgE的能力和致敏效力，并调查蛋白质糖基化对过敏原的影响。致敏效力将通过转基因植物的IgE结合和介体释放分析来评估，无论是否暴露在生物和非生物胁迫条件下。由于几个目标蛋白参与植物防御，该子项目旨在研究补偿性过敏原病原体相关蛋白的潜在上调。在RNAi方法针对基本细胞功能的情况下，将选择或产生具有降低致敏效力的同源蛋白(即Profilin)来替代生物功能。此外，将通过检测特定的过敏原和糖基化反应的过敏原多肽来研究食品加工过程中发生的修饰对小鼠的过敏原效力、T细胞反应性和IgE诱导能力的影响。此外，还将合成单独的糖基化化合物，以便随后与选定的目标蛋白质和产品进行共价偶联，并使用相同的方法进行分析。