EVOBOOSTER - Impact of transposable elements on gene regulatory networks: application to fast-evolving biological pathways in fish

EVOBOOSTER - 转座元件对基因调控网络的影响：应用于鱼类快速进化的生物途径

• 批准号: 316652466
• 负责人: Professor Dr. Manfred Schartl, Ph.D.
• 金额: --
• 依托单位: Lehrstuhl für Entwicklungsbiochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/316652466?language=en
• 关键词: EVOBOOSTER; Impact; transposable; elements; gene

Because of their repeated and mobile nature, transposable elements have the potential to boost evolution through the fast spreading of ready-to-use regulatory sequences that can generate new gene regulations and rewire host regulatory networks, this potentially promoting rapid diversity, adaptation and speciation. Fish represent a very attractive model to test this hypothesis, since they show a high level of diversity affecting almost all facets of their biology. In addition, fish contain in their genomes many more families of transposable elements than mammals and other vertebrates. In this project, we aim to investigate the impact of transposable elements on sexual development and pigmentation, which are both hypervariable in fish. We will systematically identify and analyze at the functional level cis-regulatory sequences that are associated with genes expressed in male and female gonads or during the development of normal and malignant pigment cells, or that are bound by transcription factors with major functions in sexual development and pigmentation. Regulatory non-coding RNAs derived from transposable elements and expressed in gonads and pigment cells will also be identified and studied. This project, which involves two teams with complementary expertise (Partner 1, M Schartl, Würzburg: biology of sexual development and pigmentation; Partner 2, JN Volff, Lyon: transposable elements and genome variability) will shed a new light on the role of the mobilome on regulatory variability in living organisms, with an assessment of the potential role of transposable elements to promote rapid speciation and adaptive responses to environmental changes.

由于其重复和移动的性质，转座因子有可能通过快速传播现成的调节序列来促进进化，这些序列可以产生新的基因调控和重新连接宿主调控网络，这可能促进快速的多样性、适应性和物种形成。鱼类代表了一个非常有吸引力的模型来验证这一假设，因为它们表现出高度的多样性，影响着它们生物学的几乎所有方面。此外，鱼类的基因组中含有比哺乳动物和其他脊椎动物更多的转座因子家族。在这个项目中，我们旨在研究转座因子对鱼类性发育和色素沉着的影响，这两个因素在鱼类中都是高度可变的。我们将在功能水平上系统地识别和分析与男性和女性性腺或正常和恶性色素细胞发育过程中表达的基因相关的顺式调控序列，或与性发育和色素沉着主要功能的转录因子结合的顺式调控序列。来自转座元件并在性腺和色素细胞中表达的调节性非编码rna也将被鉴定和研究。该项目涉及两个具有互补专业知识的团队（合作伙伴1，M Schartl, w<s:1> rzburg：性发育和色素沉积生物学；合作伙伴2，JN Volff， Lyon：转座因子和基因组变异性），将揭示生物体内移动组在调节变异性中的作用，评估转座因子在促进快速物种形成和对环境变化的适应性反应中的潜在作用。