Quantitative analysis of the cellular growth dynamics during lateral plant growth

植物侧生过程中细胞生长动力学的定量分析

• 批准号: 356728708
• 负责人: Professor Dr. Thomas Greb
• 金额: --
• 依托单位: Centre for Organismal Studies (COS)
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/356728708?language=en
• 关键词: Quantitative; analysis; cellular; growth; dynamics

Organ expansion represents a fascinating example of cellular coordination during the development of multicellular organisms. Lateral growth of plant shoot and root axes is mediated by the cambium, a group of stem cells producing very rigid tissues in a bidirectional manner. In this project, we leverage the anatomical features of cambium-based organ growth and probe the role of mechanical forces applied by surrounding tissues in instructing stem cell behavior. Based on preliminary data from the first funding period obtained by computational modelling and genetic approaches altering cell wall rigidity we hypothesize that mechanical stress generated by differentiating xylem cells, influence the division orientation of cambium stem cells. In the project, a PhD student and a postdoc will test this hypothesis by targeted modulations of cell wall properties and of microtubule regulation, in silico modelling of inter-tissue forces and Brillouin spectroscopy. Thereby and by being firmly embedded into the current consortium, the project explores a fundamental aspect of organ morphogenesis and contributes to the discovery of general morphodynamic principles.

器官扩张是多细胞生物体发展过程中细胞协调的一个有趣的例子。植物地上部和根轴的侧向生长是由形成层调节的，形成层是一组以双向方式产生非常坚硬的组织的干细胞。在这个项目中，我们利用基于形成层的器官生长的解剖学特征，并探索周围组织施加的机械力在指导干细胞行为中的作用。根据通过计算模型和改变细胞壁硬度的遗传方法获得的第一个资助期的初步数据，我们假设木质部细胞分化产生的机械应力影响形成层干细胞的分裂方向。在该项目中，一名博士生和一名博士后将通过对细胞壁属性和微管调节的定向调制，在组织间力和布里渊光谱的电子模拟中来检验这一假说。因此，通过牢牢地嵌入目前的联合体，该项目探索了器官形态发生的一个基本方面，并有助于发现一般的形态动力学原理。