Connectomic analysis of an entire Drosophila: connecting the central nervous system (CNS) with the periphery in the larval feeding system

整个果蝇的连接组学分析：连接中枢神经系统（CNS）与幼虫进食系统的外围

• 批准号: 388367495
• 负责人: Professor Dr. Michael Pankratz
• 金额: --
• 依托单位: Abteilung Molekulare Hirnphysiologie und Verhaltensforschung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/388367495?language=en
• 关键词: Connectomic; analysis; entire; Drosophila; connecting

A major achievement in the neurosciences would be a complete connectivity map of a brain, together with a functional knowledge of what these circuits do at a behavioral and physiological level. We have been involved in the reconstruction of the Drosophila larval brain at synaptic resolution, focusing on the sensory-motor circuits that underlie feeding behavior. We have recently provided a synaptic connectome of a group of peptidergic neurons in the subesophageal zone involved in modulating food intake, and have shown that it connects chemosensory inputs with endocrine outputs. Our current aim is a reconstruction of the motor circuits comprising the feeding network. We will first trace all axons of the pharyngeal nerves involved in food intake and map their sensory projection domains in the central nervous system. We will then identify all motor neurons of these nerves, which innervate muscles involved in pharyngeal pumping and mouth hook movements, and identify their presynaptic partners. The motor output analysis will also be extended to map all neurosecretory neurons targeting the major endocrine center, the ring gland. These studies will be complemented with functional analysis on selected neurons, as well as a functional imaging screen of known neurotransmitter positive populations for their involvement in starvation response. In sum, we hope to provide a comprehensive connectivity map of the sensory-motor circuits in the brain that underlie feeding regulation.

神经科学的一项重大成就将是绘制出完整的大脑连接图，以及这些回路在行为和生理层面上的功能知识。我们在突触分辨率上参与了果蝇幼虫大脑的重建，重点研究了觅食行为背后的感觉-运动回路。我们最近在食管亚区提供了一组肽能神经元的突触连接组，这些神经元参与调节食物摄入，并表明它将化学感觉输入与内分泌输出联系起来。我们目前的目标是重建包含馈电网络的电机电路。我们将首先追踪参与食物摄取的咽神经的所有轴突，并绘制其在中枢神经系统中的感觉投射域。然后，我们将识别这些神经的所有运动神经元，这些神经支配参与咽泵和口钩运动的肌肉，并识别它们的突触前伙伴。运动输出分析也将扩展到映射所有针对主要内分泌中心，环腺的神经分泌神经元。这些研究将辅以对选定神经元的功能分析，以及对已知参与饥饿反应的神经递质阳性群体进行功能成像筛查。总之，我们希望提供一个全面的连接图，在大脑的感觉-运动电路的进食调节基础。