Molecular analysis of genes controlling food intake and growth in Drosophila

控制果蝇食物摄入和生长的基因的分子分析

• 批准号: 5251206
• 负责人: Professor Dr. Michael Pankratz
• 金额: --
• 依托单位: Abteilung Molekulare Hirnphysiologie und Verhaltensforschung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2000
• 资助国家: 德国
• 起止时间: 1999-12-31 至 2004-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5251206?language=en
• 关键词: Molecular; analysis; genes; controlling; food

The proposal addresses the question how food intake and growth are coordinated in a multicellular organism. Using Drosophila as a genetically accessible system, we are interested in identifying the molecular signals that control food intake, which organs they orginate from and act on, and how their activities are regulated in response to differing growth conditions. In a genetic screen for mutants that are defective in feeding, we have identified three genes that are required for proper food intake and growth of the larva. The first gene we have molecularly characterized is pumpless, which encodes an enzyme involved in glycine catabolism and which is expressed exclusively in the fat body. We have proposed a model in which amino acid dependent signal(s) arising from the fat body induces cessation of feeding in the larva; this signaling system may also mediate growth transition from larval to the pupal stage during Drosophila development. The goal of the proposed projects is to investigate this model and to molecularly characterize the other two genes that have been identified. The molecular cloning of these genes, combined with a detailed biochemical and genetic analysis of the gene products, would be a first step in providing mechanistic insights into how an organism coordinates growth pattern with food intake behavior.

该提案解决了多细胞生物体中食物摄入和生长如何协调的问题。使用果蝇作为一个遗传可访问的系统，我们感兴趣的是确定控制食物摄入的分子信号，它们来自哪些器官并作用于哪些器官，以及它们的活动如何响应不同的生长条件进行调节。在对摄食缺陷突变体的遗传筛选中，我们已经确定了三个基因，这三个基因是幼虫正常摄食和生长所必需的。第一个基因，我们有分子特征是pumpless，它编码的酶参与甘氨酸catalysts和表达专门在脂肪体。我们已经提出了一个模型，在该模型中，由脂肪体产生的氨基酸依赖性信号（S）诱导幼虫停止进食，该信号系统也可能介导果蝇发育过程中从幼虫到蛹阶段的生长过渡。拟议项目的目标是调查这一模型，并从分子上表征已确定的其他两个基因。这些基因的分子克隆，结合基因产物的详细生化和遗传分析，将是提供有机体如何协调生长模式与食物摄入行为的机制见解的第一步。