权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Treponemes in wild lagomorphs: Genetic diversity and relatedness to human pathogenic T. pallidum

野生兔类中的密螺旋体：遗传多样性及其与人类致病性梅毒螺旋体的相关性

基本信息

批准号：
391166847
负责人：
Professor Dr. Sascha Knauf, Ph.D.
金额：
--
依托单位：
Friedrich-Loeffler-Institut - Bundesforschungsinstitut für Tiergesundheit (FLI)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2018
资助国家：
德国
起止时间：
2017-12-31 至 2021-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/391166847?language=en
关键词：
Treponemes wild lagomorphs Genetic diversity

项目摘要

Bacteria of the genus Treponema are known human and animal pathogens. While human and nonhuman primate pathogenic T. pallidum has been intensively studied for decades, there is little information on the diversity, clinical relevance, and occurrence of treponemes (T. paraluisleporidarum ecovar Cuniculus (TPeCuniculus) and ecovar Lepus (TPeLepus)) that naturally infect wild rabbits and hares (lagomorphs). Infection, however, is widespread in Europe. Similar to human syphilis, lagomorph treponemal disease is typically sexually transmitted and results in erythema and edema of the anogenital area, often followed by skin ulceration and crusting of the lesion. The rabbit pathogenic treponemes are not infectious for humans despite the fact, that these treponemes show genome sequence identity with human T. pallidum higher than 98%. Therefore, an analysis focused on treponemes infecting lagomorphs will reveal not only characterization of these animal pathogens but will also help to better understand the evolution and biology of human infecting T. pallidum. There are three specific aims of the submitted project including i) testing of seroprevalence of rabbit and hare treponematoses among animals caught in the Czech Republic and in Germany as well as in other selected areas mainly in Europe, ii) analysis of strain diversity within TPeCuniculus and TPeLepus as well as its correlation with high host-specificity of these treponemes, and iii) genomic analysis of the potential evolutionary ancestry of TPeLepus. In summary, we are planning to screen over 2,000 lagomorph samples taken mostly from European animals for determination of serological status and the presence of treponemal DNA. We expect to characterize about 90 DNA-containing samples for genetic diversity through Multi-Locus Strain Typing of TPeCuniculus or TPeLepus as well as we anticipate to whole genome sequence over a dozen of newly isolated lagomorph treponemes.

密螺旋体属的细菌是已知的人类和动物病原体。而人类和非人灵长类的致病性T.梅毒螺旋体的研究已经深入了几十年，但关于螺旋体的多样性、临床相关性和发生率的信息却很少（T. paraluisleporidarum ecovar Cuniculus（TPeCuniculus）和ecovar Lepus（TPeLepus）），其天然感染野兔和野兔（兔形动物）。然而，感染在欧洲很普遍。与人类梅毒类似，兔形密螺旋体病通常通过性传播，导致肛门生殖器区域的红斑和水肿，通常随后出现皮肤溃疡和病变结痂。兔致病性密螺旋体对人类没有感染性，尽管这些密螺旋体显示出与人类T.苍白球高于98%。因此，对感染兔形目动物的密螺旋体的分析不仅将揭示这些动物病原体的特征，而且将有助于更好地理解人类感染T的进化和生物学。苍白球提交的项目有三个具体目标，包括i）检测在捷克共和国和德国以及主要在欧洲的其他选定地区捕获的动物中兔和野兔密螺旋体病的血清阳性率，ii）分析TPeCuniculus和TPeLepus内的菌株多样性及其与这些密螺旋体的高宿主特异性的相关性，和iii）对天狗属的潜在进化祖先的基因组分析。总之，我们计划筛选2,000多个兔形虫样本，主要来自欧洲动物，以确定血清学状态和密螺旋体DNA的存在。我们期望通过TPeCuniculus或TPeLepus的多位点菌株分型来表征约90个含有DNA的样品的遗传多样性，并且我们期望对十几个新分离的兔形密螺旋体进行全基因组测序。