Reactivation of the inactive X chromosome through the asymmetric precursor cell division in the embryonal carcinoma cell population

通过胚胎癌细胞群中不对称前体细胞分裂重新激活失活的 X 染色体

• 批准号: 24650614
• 负责人: YOSHIDA Ikuya
• 金额: $ 2.5万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650614/
• 关键词: 幹細胞; 不活性X染色体; 再活性化; 非対称分裂; 胚性腫瘍細胞; 前駆細胞; 国際情報交換

A single-cell derived cell populations of mouse embryonic stem (ES) and embryonal carcinoma (EC) cell are not homogeneous: heterogeneous cells in phenotype and/or function are frequently appeared in these undifferentiated cell populations. However mechanistic detail of their cellular heterogeneity remains unknown. We investigated how inactive X chromosomes (Xi) are reactivated in the undifferentiated EC cell population, and found that (1) stem-cell-like precursor cells are stochastically appeared in the populations, and (2) asymmetric cell divisions of the precursor cell generates a precursor cell and a HAT resistant (= Xi-reactivated) cell. Further analysis will be needed to clarify the underlying mechanisms for the acquisition of stem-cell-like properties, including Xi-reactivation ability, during asymmetric cell divisions.

小鼠胚胎干细胞（ES）和胚胎癌细胞（EC）的单细胞来源的细胞群不是同质的：在这些未分化的细胞群中经常出现表型和/或功能异质的细胞。然而，其细胞异质性的机制细节仍然未知。我们研究了失活X染色体（Xi）在未分化EC细胞群中如何被重新激活，发现（1）干细胞样前体细胞在未分化EC细胞群中随机出现，（2）前体细胞的不对称细胞分裂产生前体细胞和HAT抗性（= Xi-重新激活）细胞。将需要进一步的分析，以澄清收购干细胞样的属性，包括Xi-再激活能力，在不对称细胞分裂的潜在机制。

项目成果

Klf4強制発現によるTsix RNAの転写誘導

通过强制表达 Klf4 诱导 Tsix RNA 转录

• 发表时间: 2013
• 作者: Kawashima M;et al.;吉田郁也
• 通讯作者: 吉田郁也

Spontaneous reactivation of the inactive X chromosome through asymmetric precursor-cell division in the mouse embryonal carcinoma cell line

通过小鼠胚胎癌细胞系中不对称前体细胞分裂自发重新激活失活的 X 染色体

• 发表时间: 2012
• 作者: Kawashima M;et al.;吉田郁也;Hiyama E;吉田郁也
• 通讯作者: 吉田郁也