Development of novel cancer specific DDS utilizing circulating microRNA-derived mechanism

我们发现巨噬细胞在CD63、CD9和Alix等外切体标志物的作用下，是高分泌的外切体样颗粒。制备CD63-GFP融合蛋白，将其克隆到Lenti病毒载体中。将CD63-GFP蓝蒂病毒感染癌细胞和巨噬细胞，建立了稳定表达CD63-GFP融合蛋白的细胞系。我们从这些细胞系中分离出外切体，并通过超速离心法进行纯化，发现这些纯化的外切体成功地衍生为受体细胞。这些结果表明，exosome可以作为一种新的microRNAs DDS的工具。

We found that macrophage is highly secreted exosome like particle with exosome maker such as CD63 and CD9 and Alix. We made CD63-GFP fusion protein and cloned into lenti-virus vector. CD63-GFP lenti-virus is infected to cancer and macrophage and established cell line stably expressing CD63-GFP fusion protein. We isolated exosome from these cell lines and purified by ultra-centrifugation, and found that these purified exosome is successfully derived to recipient cells. These results suggest that exosome can be a tools for novel DDS of microRNAs.