Evolutionary engineering based creation of monomer-supplying enzyme that allows the microbial synthesis of new monomer-incorporated polymers

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This study is based on the microbial system for production of the new type polymers by polymerizing enzyme with altered substrate specificity. So far the most fascinating achievement is the first incorporation of lactic acid as a monomer into the polymer through engineering the polymerase. Upon the project, the reactivity of CoA-transferase towards new monomers susch as lactic acid should be a key for the synthesis of monomer CoA forms. Glycolic acid is a second target monomer bearing 2-OH. Through engineering the CoA transferase as well as polymerase, the incorporation of glycolic acid into the polymeric back bone was demonstrated by using the microbial system carrying engineered enzymes. The fraction of glycolic acid was increased depending on the increase in the glycolic acid concentration.