Establishing Chymomyza costata as a new genetic model system to study photoperiodism

建立Chymomyza costata作为研究光周期现象的新遗传模型系统

• 批准号: 24657007
• 负责人: KONDO Shu
• 金额: $ 2.66万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657007/
• 关键词: モデル生物; 光周性; ショウジョウバエ; 概日リズム; 遺伝学

Photoperiodism is a mechanism by which living organisms perceive long or short day lengths and differentially respond to each condition. Although photoperiodism is common in species living in areas where there are four seasons, little has been studied on its molecular mechanisms. Here we tried to establish Chymomyza costata as a new genetically tractable model system to study photoperiodims. Although I could successufully culture C. costata in the laboratory, pair-mating and mass egg collection were found to be difficult, making C. costata an unideal species for genetic manipulation. Therefore, I turned to other Drosophila species. After comparing several different species, I found that reproductive diapause of D. bifasciata is the most ideal system to study photoperiodism. In addition, I found that seasonal color variation in D. puturida is under the photoperiodic control.

光周期是生物体感知白昼长短并对不同条件作出不同反应的一种机制。虽然光周期现象在生活在四季地区的物种中很常见，但对其分子机制的研究却很少。在此，我们试图建立一个新的可遗传处理的模式系统来研究光周期植物。虽然我在实验室中成功地培养了棘球蚴，但发现配对和大量收集卵很困难，这使得棘球蚴成为一个不理想的遗传操作物种。因此，我转向其他种类的果蝇。在比较了几种不同的物种后，我发现双歧蝶的生殖滞育是研究光周期的最理想系统。此外，我发现阴毛田鼠的季节颜色变化受光周期控制。

项目成果

Seasonal body color polyphenisms in Drosophila putrida is under photoperiodic control

腐臭果蝇的季节性体色多态性受光周期控制

• 发表时间: 2013
• 作者: Shu Kondo;Ryu Ueda
• 通讯作者: Ryu Ueda

Seasonnal body color polyphenism in Drosophila putrida is under photoperiodic control

腐臭果蝇的季节性体色多态性受光周期控制

• 发表时间: 2013
• 作者: Shu Kondo;Ryu Ueda
• 通讯作者: Ryu Ueda

Highly Improved Gene Targeting by Germline-Specific Cas9 Expression in Drosophila

• DOI: 10.1534/genetics.113.156737
• 发表时间: 2013-11-01
• 期刊: GENETICS
• 影响因子: 3.3
• 作者: Kondo, Shu;Ueda, Ryu
• 通讯作者: Ueda, Ryu