Can transgenic rootstock accelerated flowering transduce flowering signal to scion?

转基因砧木加速开花能否将开花信号转导至接穗？

• 批准号: 24658034
• 负责人: WADA Masato
• 金额: $ 2.5万
• 依托单位: National Agriculture and Food Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658034/
• 关键词: 花成; リンゴ; 台木; 接ぎ木; FT; TFL1; サイレンシング; TFL

In the signal transduction experiments of flowering on apple, transgenic apples by rolC:AtFT were rootstocks, and nontransgenic apples were scions. They were grafted with changing length of scions. For 2 years of the experiment, no flower induction of scions were observed in spite of lengths. Bud grafting has been tried, we did not have the results in this research period. MdTFL1 suppressed rootstocks by silencing were used for same grafting experiment to detect silencing signal transduction. Nontransgenic scions have not promoted flowering. In other experiment, two shoots from nontransgenic apple was grafted rolC:AtFT transgenic apple to one shoot and other shoot were removed all leaves. The shoot without leaves seemed to increase sink ability, but the shoot did not set flowers.

在苹果开花的信号转导实验中，以转rolC：AtFT基因苹果为砧木，非转基因苹果为接穗。它们用不同长度的接穗嫁接。对于2年的实验，没有观察到花诱导的接穗，尽管长度。芽接已经尝试过，本研究期内还没有结果。将通过沉默而抑制MdTFL1的砧木用于相同的嫁接实验以检测沉默信号转导。非转基因接穗没有促进开花。在另一个试验中，将转rolC：AtFT基因苹果的两个新梢嫁接到一个新梢上，另一个新梢去除所有叶片。无叶枝条似乎增加了库能力，但不开花。

项目成果

リンゴ‘はるか’における果実中デンプン含量と新梢葉の光合成速度の変化

苹果‘Haruka’果实淀粉含量及新叶光合速率的变化

• 发表时间: 2013
• 作者: 佐々木舟;渡邉学;村上政伸;佐川了;和田雅人
• 通讯作者: 和田雅人

オーキシン合成酵素遺伝子（iaaM, iaaH）およびサイトカイニン合成酵素遺伝子（ipt）を導入した形質転換体自根樹及び接木樹等の成長解析

引入生长素合酶基因（iaaM、iaaH）和细胞分裂素合酶基因（ipt）的转化自根树和嫁接树的生长分析

• 发表时间: 2013
• 作者: 鎌田詩織;李 積軍;高橋里帆;小森貞男;渡邉 学;和田雅人;壽松木 章
• 通讯作者: 壽松木 章

MdPISTILLATA抑制組換えリンゴの作出

抑制 MdPISTILLATA 的重组苹果的创建

• 发表时间: 2012
• 作者: 和田雅人;守谷(田中)友紀;本多親子;岩波宏;小森貞男;和田雅人，田中紀充，守谷(田中)友紀，本多親子，岩波宏，耳田直純，小森貞男
• 通讯作者: 和田雅人，田中紀充，守谷(田中)友紀，本多親子，岩波宏，耳田直純，小森貞男

Application of an Analog of 9, 10-ketol-octadecadienoic acid (KODA), Affected Flower Bud Formation and MdTFL1 and MdFT1 Gene Expressions in Apple Buds under Heavy-crop and Shade Conditions

• DOI: 10.21273/jashs.138.2.102
• 发表时间: 2013-03-01
• 期刊: JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
• 影响因子: 1.9
• 作者: Kittikorn, Monrudee;Okawa, Katsuya;Watanabe, Naoharu
• 通讯作者: Watanabe, Naoharu

Effects of microspore development stage and cold pre-treatment of flower buds on embryo induction in apple (Malus × domestica Borkh.) anther culture.

小孢子发育阶段和花芽冷预处理对苹果花药培养中胚诱导的影响。

• 发表时间: 2013
• 期刊: Journal of Japanese Society for Horticulture Science
• 作者: Chunfen Zhang;Tatsuro Tsukuni;Miyuku Ikeda;Mamoru Sato;Hatsuhiko Okada;Yoshitaka Ohashi;Hideyuki Matsuno;Toshiya Yamamoto;Masato Wada;Nobuyuki Yoshikawa;Shogo Matsumoto;Jijun Li;Naozumi Mimida;Manabu Watanabe;Akira Suzuki;Sadao Komori
• 通讯作者: Sadao Komori