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generation of the HCV-infectable mouse-an animal model for inflammation cancer

generation of the HCV-infectable mouse-an animal model for inflammation cancer
HCV感染小鼠的产生——炎症性癌症动物模型
批准号:
24659585
负责人:
UEZATO Tadayoshi
金额:
$2.41万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012-04-01 至 2014-03-31

项目摘要

项目成果

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中文摘要
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英文摘要
To investigate whether mouse Cd81, or Ocln inhibited the entry of HCV into mouse hepatocytes, we established the Cd81-expressing Huh7.5.1 cells and the Ocln-expressing Huh7.5.1 cells. HCVpp assay showed that no significant differences the infectivity of the virus among the parental, Cd81- and Ocln-Huh7.5.1 cells. This means that mouse Cd81 or mouse Ocln protein does not inhibit the entry process of HCV into mouse hepatocytes.Next we planned to express the large amounts of human CD81, OCLN, CLDN1 and SCAVRB1 and made the targeting vectors to obtain the homologously recombinated ES cells. We obtained 4 ES clones for CD81/OCLN expression and one clone for CLDN1/SCAVRB1.
期刊论文(10)
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会议论文
Screening of natural medicine that efficiently activate neurite outgriwth in PC12 cells in C2C12 cultured medium
筛选在C2C12培养基中有效激活PC12细胞神经突生长的天然药物
DOI: --
发表时间: 2012
期刊: Biomed Res
影响因子: --
作者: [Uezato T, Sato E, Miura N.]
通讯作者: Miura N.
Generation and characterization of Tbx1-AmCyan1 transgenic reporter mouse line that relectively labels developing thymus primordium
选择性标记发育中胸腺原基的 Tbx1-AmCyan1 转基因报告小鼠系的生成和表征
DOI: --
发表时间: 2013
期刊: Transgenic Res
影响因子: 3
作者: [Kimura W, Sharkar MT, Sultana N, Islam MJ, Uezato T, Miura N.]
通讯作者: Miura N.
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [Uezato T, Suzuki M, Sato E, Miura N.]
通讯作者: Miura N.
Generation and characterization of Tbx1-AmCyan1 transgenic reporter mouse line that selectively labels developing thymus primordium.
选择性标记发育中胸腺原基的 Tbx1-AmCyan1 转基因报告小鼠系的生成和表征。
DOI: 10.1007/s11248-012-9664-5
发表时间: 2013
期刊: Transgenic Res
影响因子: 3
作者: [Zheng YW, Tsuchida T, Shimao T, Li B, Takebe T, Zhang RR, Sakurai Y, Ueno Y, Sekine K, Ishibashi N, Imajima M, Tanaka T, Taniguchi H, 木村紘爾,大橋俊孝,中山 勝,小宮山高明,市村浩一,内藤一郎,浅野博昭,佃 和憲,内藤 稔,三好新一郎,大塚愛二,二宮善文., Kimura W et al.]
通讯作者: Kimura W et al.
9
    Effect of disturbing endocrine drug, DEHP, on microcyst formation in the epithelial cells.
    • 批准号:
      11680571
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.43万
    • 财政年份:
      1999
    • 负责人:
      UEZATO Tadayoshi
    • 依托单位:
    海外基金