generation of the HCV-infectable mouse-an animal model for inflammation cancer

HCV感染小鼠的产生——炎症性癌症动物模型

• 批准号: 24659585
• 负责人: UEZATO Tadayoshi
• 金额: $ 2.41万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659585/
• 关键词: C型肝炎ウイルス; 感染モデルマウス; ウイルス受容体; ヒト感染因子; ヒト受容体蛋白質; CD81; Scavenger receptor B1; Claudin 1; Occludin; 侵入; CLDN1; OCLN; SRBI

To investigate whether mouse Cd81, or Ocln inhibited the entry of HCV into mouse hepatocytes, we established the Cd81-expressing Huh7.5.1 cells and the Ocln-expressing Huh7.5.1 cells. HCVpp assay showed that no significant differences the infectivity of the virus among the parental, Cd81- and Ocln-Huh7.5.1 cells. This means that mouse Cd81 or mouse Ocln protein does not inhibit the entry process of HCV into mouse hepatocytes.Next we planned to express the large amounts of human CD81, OCLN, CLDN1 and SCAVRB1 and made the targeting vectors to obtain the homologously recombinated ES cells. We obtained 4 ES clones for CD81/OCLN expression and one clone for CLDN1/SCAVRB1.

为了研究小鼠Cd 81或Ocln是否抑制HCV进入小鼠肝细胞，我们建立了表达Cd 81的Huh7.5.1细胞和表达Ocln的Huh7.5.1细胞。HCVpp检测结果显示，病毒在亲本细胞、Cd 81-细胞和Ocln-Huh 7.5.1细胞中的感染性无显著差异。下一步我们计划大量表达人CD 81、OCLN、CLDN 1和SCAVRB 1，并制备靶向载体，获得同源重组ES细胞。我们获得了4个用于CD 81/OCLN表达的ES克隆和1个用于CLDN 1/SCAVRB 1的克隆。

项目成果

Screening of natural medicine that efficiently activate neurite outgriwth in PC12 cells in C2C12 cultured medium

筛选在C2C12培养基中有效激活PC12细胞神经突生长的天然药物

• 发表时间: 2012
• 期刊: Biomed Res
• 作者: Uezato T;Sato E;Miura N.
• 通讯作者: Miura N.

Generation and characterization of Tbx1-AmCyan1 transgenic reporter mouse line that relectively labels developing thymus primordium

选择性标记发育中胸腺原基的 Tbx1-AmCyan1 转基因报告小鼠系的生成和表征

• 发表时间: 2013
• 期刊: Transgenic Res
• 影响因子: 3
• 作者: Kimura W;Sharkar MT;Sultana N;Islam MJ;Uezato T;Miura N.
• 通讯作者: Miura N.

Purification and characterization of effective constituentes of Trichosanthes that promote neurite outgrowth in PC12 cells

促进 PC12 细胞神经突生长的瓜蒌有效成分的纯化和表征

• 发表时间: 2012
• 作者: Uezato T;Suzuki M;Sato E;Miura N.
• 通讯作者: Miura N.

Generation and characterization of Tbx1-AmCyan1 transgenic reporter mouse line that selectively labels developing thymus primordium.

选择性标记发育中胸腺原基的 Tbx1-AmCyan1 转基因报告小鼠系的生成和表征。

• DOI: 10.1007/s11248-012-9664-5
• 发表时间: 2013
• 期刊: Transgenic Res
• 影响因子: 3
• 作者: Zheng YW;Tsuchida T;Shimao T;Li B;Takebe T;Zhang RR;Sakurai Y;Ueno Y;Sekine K;Ishibashi N;Imajima M;Tanaka T;Taniguchi H;木村紘爾，大橋俊孝，中山 勝，小宮山高明，市村浩一，内藤一郎，浅野博昭，佃 和憲，内藤 稔，三好新一郎，大塚愛二，二宮善文．;Kimura W et al.
• 通讯作者: Kimura W et al.

Liver tumor formation by a mutant retinoblastoma protein in the transgenic mice is caused by an up-regulation of c-Myc target genes

转基因小鼠中突变视网膜母细胞瘤蛋白形成的肝脏肿瘤是由 c-Myc 靶基因上调引起的

• 发表时间: 2012
• 期刊: Biochem Biophys Res Commun
• 作者: Wang B;Hikosaka K;Sultana N;Sharkar MTK;Noritake H;Kimura W;Wu Y-X;Kobayashi Y;Uezato T;Miura N
• 通讯作者: Miura N