Preventive measurement for vaccine brak of Marek's Disease, Divelopment of the vaccine in particular

本研究的最终目的是研制马立克氏病疫苗，防止马立克氏病引起的疫苗破损。1)火鸡马立克氏病病毒(MDV)和火鸡疱疹病毒(HVT)在血清学上分为3组。许多杂交瘤细胞分泌针对每一群或菌株的单抗作为接种。通过使用这些抗体，有可能鉴定从该领域分离的病毒的血清型。在此基础上，研制了几种针对MD淋巴瘤细胞的单抗，用于MD肿瘤细胞的鉴定。2)研究了MD在日本鹌鹑中的发病率和疫苗接种效果。在许多鹌鹑身上观察到了MD特有的病理损伤。淋巴瘤性改变的发生率与毛囊中MMV特异性抗原的出现呈正相关，而淋巴瘤性改变的发生率与人群中抗HVT抗体的阳性率呈负相关。从禽类中分离到的MDV属于1型血清，对鸡具有较强的致病性。3)新型非致癌马立克氏病病毒的分离是研制马立克氏病疫苗、控制马立克氏病疫苗破损的关键。人们试图从13只鸡和10只野鸡身上分离出该病毒。从TH鸡的细胞中分离到13株病毒，经单抗鉴定均为2型病毒，利用这些病毒可在不久的将来研制出更有效的抗MD疫苗。

The final aim of the present study is to develop Marek's disease (MD) vaccine which prevents the vaccine break induced by MD. The following results were obtained at Present.1) The group of MD virus (MDV) and herpesvirus of turkey (HVT) is serologically devided into 3 groups. Many hybridoma secreting monoclonal antibody specific to each group or strain used as inoculum were developed. By using these antibodies, it became possible to identify the serotype of the viruses isolated from th field. Further, several monoclonal antibodies specific to MD lymphoma cells were developed for identification of MD tumor cells.2) The incidence of MD and effect of HVT vaccine in Japanese quails were investigated. Pathological lesions specific to MD were observed in many quails. There was a positive correlation between the incidence of lymphomatous changes and the prosence of MMV-specific antigen in feather follicle, and there was a negative correlation between the incidence of lympomatous changes and the presence of antibody against HVT on a flock basis. the MDV isolated from the quails belongs to serotype 1 and these were highly pathogenic to chickens. the similar virus was also isolated from chikens in field.3) It is essential to isolate the new type of non-oncogenic MDV for development of the MD vaccine to control the vaccine break of MD. Attempts were made to isolate the virus from 13 chickens and 10 pheasant. Thirteen viruses isolated from cell of th chickens, and all of the viruses belong to serotype 2 as identified by the monoclonal antibody.By using these viruses, it is feasible to develop the more effective vaccine fought against vaccine break of MD in the near future.