Development of polyvalent recombinant vaccines for chickens and companion animals

鸡和伴侣动物多价重组疫苗的开发

• 批准号: 04556044
• 负责人: MIKAMI Takeshi
• 金额: $ 11.2万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04556044/
• 关键词: Recombinant vaccine; Vector virus; Feline herpesvirus type 1 (FHV-1); Marek's disease virus (MDV); Fowlpox virus (FPV); Newcastle disease virus (NDV); Chicken infections bursal disease virus (IBDV); Feline calicivirus (FCV); FHV-1gB; FHV-1gD; リン

The aim of the present studies is to develop polyvalent recombinant (rec) vaccines which are superior to safeness, inexpensiveness and effectiveness to chickes and companion animals, and to put the vaccines to practical use. The following results are obtained.1. Development of vector viruses for polyvalent rec vaccines.Fowlpox virus (FPV), Marek's disease virus serotype 1 (MDV-1) and feline herpesvirus type 1 (FHV-1) were found to be useful as vector viruses.2. Identification and expression of various virus genes encoding proteins which are related to protective antigens in infection.We identified a segment A gene encoding VP2 and VP3 of chicken infectious bursal disease virus (IBDV), F and HN genes of Newcastle disease virus (NDV), gB,gC,gD and thymidine kinase (TK) genes of FHV-1, gB,gC and gD genes of MDV-1, a capsid gene of feline calicivirus (FCV) and gag and env genes of feline immunodeficiency virus (FIV). We succeeded in expressing most of the genes.3. Protective ability of the gene products in vivo.Chickens inoculated with rec FPV inserted the F and HN genes and rec MDV-1 inserted the F gene of NDV elicited immune response to the gene procducts as well as vector viruses and were protected against virulent NDV,FPV and MDV in challenge experiments.4. Expression of protective antigen in Baculovirus system and development of polyvalent component vaccine.F and HN gene products of NDV and gB gene product to MDV showed the protective effects against NDV and MDV infections in chickins.

本研究的目的是开发对雏鸡和伴侣动物安全、廉价且有效的多价重组（rec）疫苗，并将该疫苗投入实际应用。得到如下结果： 1．多价rec疫苗载体病毒的开发。发现鸡痘病毒(FPV)、马立克氏病病毒血清型1(MDV-1)和猫疱疹病毒1型(FHV-1)可用作载体病毒。2．编码感染中保护性抗原相关蛋白的多种病毒基因的鉴定和表达。我们鉴定了编码鸡传染性法氏囊病病毒（IBDV）VP2和VP3的A段基因，新城疫病毒（NDV）的F和HN基因，gB，gC，gD和FHV-1，gB，gC和胸苷激酶（TK）基因。 MDV-1的gD基因、猫杯状病毒(FCV)的衣壳基因以及猫免疫缺陷病毒(FIV)的gag和env基因。我们成功表达了大部分基因。3．基因产物的体内保护能力。接种rec FPV的鸡插入了F和HN基因，rec MDV-1插入了NDV的F基因，在攻毒实验中对基因产物和载体病毒产生了免疫反应，对强毒NDV、FPV和MDV具有保护作用。 4．杆状病毒系统中保护性抗原的表达及多价组分疫苗的研制。NDV的F、HN基因产物和MDV的gB基因产物对雏鸡表现出对NDV和MDV感染的保护作用。

项目成果

Kohmoto,M.et al.: "Comparison of biological properties of feline immunodeficiency virus isolates using recombinant chimeric viruses." J.Gen.Virol.75. 1935-1942 (1994)

Kohmoto,M.等人：“使用重组嵌合病毒比较猫免疫缺陷病毒分离株的生物学特性。”

Ono,M.et al.: "The restriction endonuclease map of Marek's disease virus(MDV)serotype 2 and collinear relationship among three serotypes of MDV." Virology. 191. 459-463 (1992)

Ono,M.等人：“马立克氏病病毒（MDV）血清型 2 的限制性内切酶图谱以及 MDV 三种血清型之间的共线关系。”

Kamiya,N.et al.: "Protective effect of individual glycoproteins of Newcastle disease virus expressed in insect cells:the fusion protein derived from an avirulent strain had lower protective efficacy." Virus Research. 32. 373-386 (1994)

Kamiya,N.等人：“昆虫细胞中表达的新城疫病毒单个糖蛋白的保护作用：源自无毒株的融合蛋白的保护作用较低。”

Niikura, M.et al.: "Expression of the Marek's disease virus (MDV) homelogue of glycoprotein B of herpes simplex virus by a recombinant baculovirus and its identification as the B antigen (gp100,60,49) of MDV." J.Virology. 66. 2631-2638 (1992)

Niikura, M.等人：“通过重组杆状病毒表达单纯疱疹病毒糖蛋白 B 的马立克氏病病毒 (MDV) 同源物，并将其鉴定为 MDV 的 B 抗原 (gp100,60,49)。”

Niikura,M.et al.: "Expression of the Marek's disease virus(MDV)homologue of glycoprotein B of herpes simplex virus by a recombinant baculovirus and its identification as the B antigen(gp100,60,49)of MDV." Journal of Virology. 66. 2631-2638 (1992)

Niikura,M.等人：“通过重组杆状病毒表达单纯疱疹病毒糖蛋白 B 的马立克氏病病毒 (MDV) 同源物，并将其鉴定为 MDV 的 B 抗原 (gp100,60,49)。”