Development of polyvalent recombinant vaccines for chickens and companion animals

点击翻译按钮获取中文摘要

The aim of the present studies is to develop polyvalent recombinant (rec) vaccines which are superior to safeness, inexpensiveness and effectiveness to chickes and companion animals, and to put the vaccines to practical use. The following results are obtained.1. Development of vector viruses for polyvalent rec vaccines.Fowlpox virus (FPV), Marek's disease virus serotype 1 (MDV-1) and feline herpesvirus type 1 (FHV-1) were found to be useful as vector viruses.2. Identification and expression of various virus genes encoding proteins which are related to protective antigens in infection.We identified a segment A gene encoding VP2 and VP3 of chicken infectious bursal disease virus (IBDV), F and HN genes of Newcastle disease virus (NDV), gB,gC,gD and thymidine kinase (TK) genes of FHV-1, gB,gC and gD genes of MDV-1, a capsid gene of feline calicivirus (FCV) and gag and env genes of feline immunodeficiency virus (FIV). We succeeded in expressing most of the genes.3. Protective ability of the gene products in vivo.Chickens inoculated with rec FPV inserted the F and HN genes and rec MDV-1 inserted the F gene of NDV elicited immune response to the gene procducts as well as vector viruses and were protected against virulent NDV,FPV and MDV in challenge experiments.4. Expression of protective antigen in Baculovirus system and development of polyvalent component vaccine.F and HN gene products of NDV and gB gene product to MDV showed the protective effects against NDV and MDV infections in chickins.