Development of polyvalent recombinant vaccines for chickens and companion animals

鸡和伴侣动物多价重组疫苗的开发

• 批准号: 04556044
• 负责人: MIKAMI Takeshi
• 金额: $ 11.2万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04556044/
• 关键词: Recombinant vaccine; Vector virus; Feline herpesvirus type 1 (FHV-1); Marek's disease virus (MDV); Fowlpox virus (FPV); Newcastle disease virus (NDV); Chicken infections bursal disease virus (IBDV); Feline calicivirus (FCV); FHV-1gB; FHV-1gD; リン

The aim of the present studies is to develop polyvalent recombinant (rec) vaccines which are superior to safeness, inexpensiveness and effectiveness to chickes and companion animals, and to put the vaccines to practical use. The following results are obtained.1. Development of vector viruses for polyvalent rec vaccines.Fowlpox virus (FPV), Marek's disease virus serotype 1 (MDV-1) and feline herpesvirus type 1 (FHV-1) were found to be useful as vector viruses.2. Identification and expression of various virus genes encoding proteins which are related to protective antigens in infection.We identified a segment A gene encoding VP2 and VP3 of chicken infectious bursal disease virus (IBDV), F and HN genes of Newcastle disease virus (NDV), gB,gC,gD and thymidine kinase (TK) genes of FHV-1, gB,gC and gD genes of MDV-1, a capsid gene of feline calicivirus (FCV) and gag and env genes of feline immunodeficiency virus (FIV). We succeeded in expressing most of the genes.3. Protective ability of the gene products in vivo.Chickens inoculated with rec FPV inserted the F and HN genes and rec MDV-1 inserted the F gene of NDV elicited immune response to the gene procducts as well as vector viruses and were protected against virulent NDV,FPV and MDV in challenge experiments.4. Expression of protective antigen in Baculovirus system and development of polyvalent component vaccine.F and HN gene products of NDV and gB gene product to MDV showed the protective effects against NDV and MDV infections in chickins.

本研究的目的是研制出对鸡和伴侣动物安全、经济、有效的上级多价重组（rec）疫苗，并将其投入实际应用。得到以下结果.鸡痘病毒（FPV）、马立克氏病病毒血清1型（MDV-1）和猫疱疹病毒1型（FHV-1）可作为多价重组疫苗的载体病毒.本研究克隆了鸡传染性法氏囊病病毒（IBDV）的A段VP 2和VP 3基因，纽卡斯尔病病毒（NDV）的F和HN基因，FHV-1的gB、gC、gD和TK基因，MDV-1的gB、gC和gD基因，猫杯状病毒（FCV）的衣壳基因和猫免疫缺陷病毒（FIV）的gag和env基因。我们成功地表达了大部分基因。基因产物的体内保护性试验结果表明，插入NDV F和HN基因的rec FPV和插入NDV F基因的rec MDV-1接种鸡后，均能引起对基因产物和载体病毒的免疫应答，在攻毒试验中对NDV、FPV和MDV强毒株均具有保护作用. NDV的F、HN基因产物和MDV的gB基因产物在杆状病毒系统中表达保护性抗原，研制多价组分疫苗，对NDV和MDV感染鸡有保护作用。

项目成果

Kohmoto,M.et al.: "Comparison of biological properties of feline immunodeficiency virus isolates using recombinant chimeric viruses." J.Gen.Virol.75. 1935-1942 (1994)

Kohmoto,M.等人：“使用重组嵌合病毒比较猫免疫缺陷病毒分离株的生物学特性。”

Ono,M.et al.: "The restriction endonuclease map of Marek's disease virus(MDV)serotype 2 and collinear relationship among three serotypes of MDV." Virology. 191. 459-463 (1992)

Ono,M.等人：“马立克氏病病毒（MDV）血清型 2 的限制性内切酶图谱以及 MDV 三种血清型之间的共线关系。”

Kamiya,N.et al.: "Protective effect of individual glycoproteins of Newcastle disease virus expressed in insect cells:the fusion protein derived from an avirulent strain had lower protective efficacy." Virus Research. 32. 373-386 (1994)

Kamiya,N.等人：“昆虫细胞中表达的新城疫病毒单个糖蛋白的保护作用：源自无毒株的融合蛋白的保护作用较低。”

Niikura, M.et al.: "Expression of the Marek's disease virus (MDV) homelogue of glycoprotein B of herpes simplex virus by a recombinant baculovirus and its identification as the B antigen (gp100,60,49) of MDV." J.Virology. 66. 2631-2638 (1992)

Niikura, M.等人：“通过重组杆状病毒表达单纯疱疹病毒糖蛋白 B 的马立克氏病病毒 (MDV) 同源物，并将其鉴定为 MDV 的 B 抗原 (gp100,60,49)。”

Niikura,M.et al.: "Expression of the Marek's disease virus(MDV)homologue of glycoprotein B of herpes simplex virus by a recombinant baculovirus and its identification as the B antigen(gp100,60,49)of MDV." Journal of Virology. 66. 2631-2638 (1992)

Niikura,M.等人：“通过重组杆状病毒表达单纯疱疹病毒糖蛋白 B 的马立克氏病病毒 (MDV) 同源物，并将其鉴定为 MDV 的 B 抗原 (gp100,60,49)。”