The Role of Tumor-Associated Antigen in Tumor Immunity

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The purpose of this study is to investigate the role of Marek's disease tumor-associated surface antigen (MATSA) in tumor immunity. Various kinds of antigens are exprssed OSR on the cell surface of Marek's disease (MD) lymphoblastoid. cell lines, including MD tumor-associated surface antigen (MATSA),chicken fetal antigen (CFA), thrombocyte antigen, and other antigens. The results are summarized as follows :1). Natural killer (NK) activity was significantly reduced in chickens treated with CFA purified from extract of transplantable Marek's disease tumor (MSB1-clo. 18 cells). Further, the NK activity was also reduced by treatment of spleen cells with CFA in vitro.2). MATSA was solubilized from MSB1-clo. 18 cells by treatment with 0.5% Nonidet P-40, and purified by affinity chromatography coupling with monoclonal antibody (MAb) 2B9 developed against MATSA and further by ion exchange chromatography on DEAE. The MATSA had the chemical nature of a glycoprotein.3). The MAb which is reactive with both MD tumor and normal chicken thrombocytes inhibited the growth of MSB1-clo. 18 cells in vivo and in vitro.4). A panel of MAbs with specificity for chicken lymphocyte surface antigens was established. Three MAbs reacted preferentially with thymocyte, however, none of them reacted with MD derived T lymphoblastoid cell lines. Four MAbs reacted with spleen cells and peripheral blood leukocytes as well as thymocyte.