Studies on control of animal protozoan diseases by recombinant herpesvirus vector vaccines

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1) Development of animal herpesvirus vectorsIn the present study, some virulent and non-essential genes, such as TK, gE and gI genes of herpesviruses were identified and cloned. Herpesviruses deleting these genes can grow in vitro, however their virulence reduced greatly in vivo. Therefore, these genes were proposed as targets for insertion of foreign immunogenic genes.2) Cloning of genes encoding immunodominant antigens of protozoaMany genes encoding immunodominant antigens of protozoa were identified, cloned and characterized. The main genes are as follows: B. caballi P18, P48, P104; B. equi P30, P34, P82; B. gibsoni P29, P50, P130; N. caninum P36, P43; T. gondii P22, P30, P43, P54.3) Construction of recombinant herpesviruses expressing protozoan antigensRecombinant canine herpesvirus (CHV) expressing immunogenic antigens (P36, P43) of N. caninum, feline herpesvirus type 1 (FHV-1) expressing immunogenic antigens (P22, P30, P43, P54) of T. gondii, and bovine herpesvirus type 1 (BHV-1) expressing immunogenic antigen (P23) of C. parvum were constructed, and demonstrated that the recombinant antigens expressed by herpesviruses were similar to the native antigens from parasites in structure and antigenicity.4) Protective effects of recombinant vaccinesDogs immunized with recombinant CHV/P43, cats immunized with FHV-1/P54, and rabbits immunized with BHV-1/CpP23 produced a partial protective immunity against N. caninum, T. gondii, and C. parvum infections, respectively.