Studies on control of animal protozoan diseases by recombinant herpesvirus vector vaccines

重组疱疹病毒载体疫苗防治动物原虫病的研究

• 批准号: 11356009
• 负责人: MIKAMI Takeshi
• 金额: $ 24.76万
• 依托单位: Nihon University (2001-2002)Obihiro University of Agriculture and Veterinary Medicine (1999-2000)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11356009/
• 关键词: herpe virus vector; recombinant vaccine; T. gondii; N. caninum; C. parvum; B. caballi; B. equi; B. gibsoni; N.caninum; ヘルペスウイルス; ベクターワクチン; C.parvam; B.epui; T.congolense

1) Development of animal herpesvirus vectorsIn the present study, some virulent and non-essential genes, such as TK, gE and gI genes of herpesviruses were identified and cloned. Herpesviruses deleting these genes can grow in vitro, however their virulence reduced greatly in vivo. Therefore, these genes were proposed as targets for insertion of foreign immunogenic genes.2) Cloning of genes encoding immunodominant antigens of protozoaMany genes encoding immunodominant antigens of protozoa were identified, cloned and characterized. The main genes are as follows: B. caballi P18, P48, P104; B. equi P30, P34, P82; B. gibsoni P29, P50, P130; N. caninum P36, P43; T. gondii P22, P30, P43, P54.3) Construction of recombinant herpesviruses expressing protozoan antigensRecombinant canine herpesvirus (CHV) expressing immunogenic antigens (P36, P43) of N. caninum, feline herpesvirus type 1 (FHV-1) expressing immunogenic antigens (P22, P30, P43, P54) of T. gondii, and bovine herpesvirus type 1 (BHV-1) expressing immunogenic antigen (P23) of C. parvum were constructed, and demonstrated that the recombinant antigens expressed by herpesviruses were similar to the native antigens from parasites in structure and antigenicity.4) Protective effects of recombinant vaccinesDogs immunized with recombinant CHV/P43, cats immunized with FHV-1/P54, and rabbits immunized with BHV-1/CpP23 produced a partial protective immunity against N. caninum, T. gondii, and C. parvum infections, respectively.

1)动物疱疹病毒载体的研制本研究对疱疹病毒的TK、gE和gI等毒力基因和非必需基因进行了鉴定和克隆。缺失这些基因的疱疹病毒可以在体外生长，但其在体内的毒力大大降低。2）原生动物免疫显性抗原编码基因的克隆许多原生动物免疫显性抗原编码基因已被鉴定、克隆和鉴定。主要基因为：B. caballi P18、P48、P104;拟黑粉菌B.马属P30、P34、P82; B. gibsoni P29、P50、P130;犬P36、P43; T.表达弓形虫P22、P30、P43、P54抗原的重组犬疱疹病毒（CHV）的构建表达弓形虫P36、P43抗原的重组犬疱疹病毒（CHV）的构建猫疱疹病毒1型（FHV-1）表达T.表达弓形虫免疫原性抗原（P23）的牛疱疹病毒1型（BHV-1）。4）重组疫苗的免疫效果CHV/P43免疫犬、FHV-1/P54免疫猫和BHV-1/CpP 23免疫兔，均产生了抗细小疟原虫的部分保护性免疫。caninum，T. gondii和C.感染，分别。

项目成果

Umehashi, M.et al.: "Post-exposure treatment of cats with mouse-cat chimeric antibodies against feline herpesvirus type 1 and feline calicivirus"J.Vet.Med.Sci.. 64. 1673-1679 (2002)

Umehashi, M.等人：“用针对猫疱疹病毒 1 型和猫杯状病毒的小鼠-猫嵌合抗体对猫进行暴露后治疗”J.Vet.Med.Sci. 64. 1673-1679 (2002)

Sato, E., Yokoyama, N., Miyazawa, T., Maeda, K., Ikeda, Y., Nishimura, Y., Fujita, K., Kohmoto, M., Takahashi, E., and Mikami, T.: "Efficient expression of the envelope protein of feline immunodeficiency virus in a recombinant feline herpesvirus type 1 (F

Sato, E.、Yokoyama, N.、Miyazawa, T.、Maeda, K.、Ikeda, Y.、Nishimura, Y.、Fujita, K.、Kohmoto, M.、Takahashi, E. 和 Mikami, T.

Mishima, M., et al.: "Construction of recombinant feline herpesvirus type 1 expressing Toxoplasma gondii surface antigen 1"Mol.Biochem.Parasitol.. 117・1. 103-106 (2001)

Mishima，M.等人：“表达弓形虫表面抗原1的重组猫疱疹病毒1型的构建”Mol.Biochem.Parasitol..117・1(2001)。

Nishikawa, Y., Kousaka, Y., Fukumoto, S., Xuan, X., Nagasawa, H., Igarashi, I., Fujisaki, K., Otsuka, H., and Mikami, T.: "Delivery of Neospora caninum surface protein, NcSRS2 (Nc-p43), to mouse using recombinant vaccinia virus"Parasitol. Res.. 86. 934-93

Nishikawa, Y.、Kousaka, Y.、Fukumoto, S.、Xuan, X.、Nagasawa, H.、Igarashi, I.、Fujisaki, K.、Otsuka, H. 和 Mikami, T.：“新孢子虫的交付

Nishikawa, Y., Inoue, N., Xuan, X., Nagasawa, H., Igarashi, I., Fujisaki, K., Otsuka, H., and Mikami, T.: "Protective efficacy of vaccination by recombinant vaccinia virus against Neospora caninum infection"Vaccine. 19. 1381-1390 (2001)

Nishikawa, Y.、Inoue, N.、Xuan, X.、Nagasawa, H.、Igarashi, I.、Fujisaki, K.、Otsuka, H. 和 Mikami, T.：“重组痘苗病毒疫苗接种的保护功效