Development of Laser Photoacoustic Imaging Immunoassay Metchod and System

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1)Instrumentation of Laser Photoacoustic Imaging AnalyzerThe optics of laser photoacoustic microscopy with resolution of 12 <micrn>m was assembled in out lab. which has two alternative laser sources of He-Ne(633nm) and Ar ion(514.5-488nm). Microcomputer was programmed to make scanning od the cell table in X-Y direction and to get the signal through A/D converter. The data points thus obtained are 10000-30000 in number. Using sigmoidal calibration curve, the photoacoustic signal was converted to the amount og the rarget material (human <lambda>-type L-chain). the setting of the accumulating area and the classigication of the amount by coloe were also possible by this new apparatus.2)Development of new immunoassay(1)photoacounstic imaging immunoassayImmuno-enzyme reaction was used for staining. The cross sections of human embryo tissues (spleen and pancreas(5 um thick) were analyzed with 40 <micrn>m resolution. The local and total amounts of the <lambda>-type L-chain were estimated.(2)Multi-item photoacoustic immunoassayHuman <lambda>-type L-chain,<kappa>-type L-chain, and igG were analyzed by the multi-item immuno-reaction test paper which was developed by this study. The determination range was 100nm/ml-100ug/ml. the kit system for this method are under the investigation.3)New Study Field Incidentally Occurred by This ResearchUsing this apparatus. X-ray photoacoustic imaging analysis was developed. This method will open the new field of 3-dimensional photoacoustic mictoanalysis.