Development of Laser Photoacoustic Imaging Immunoassay Metchod and System

1)激光光声成像分析仪的研制在实验室组装了分辨率为12 μ m的激光光声显微镜光学系统<micrn>。其具有He-Ne（633 nm）和Ar离子（514.5- 488 nm）两种交替的激光源。微机编程，对细胞台进行X-Y方向扫描，通过A/D转换器得到信号。由此获得的数据点的数量为10000-30000。使用S形校准曲线，将光声信号转换为目标材料（人<lambda>型L链）的量。2）新型免疫分析方法的建立（1）光声成像免疫分析方法采用免疫酶反应染色。以40 μ m分辨率分析人胚胎组织（脾和胰腺（5 μ m厚））的横截面<micrn>。估算<lambda>了β-型L-链的局部含量和总量。（2）多项目光声免疫分析<lambda>方法：<kappa>用本研究研制的多项目免疫反应试纸条对人型L链、人型L链和IgG进行分析。测定范围为100 nm/ml ~ 100 μ g/ml。3）本研究附带的新的研究领域使用本仪器。发展了X射线光声成像分析技术。该方法为三维光声显微分析开辟了新的领域。

1)Instrumentation of Laser Photoacoustic Imaging AnalyzerThe optics of laser photoacoustic microscopy with resolution of 12 <micrn>m was assembled in out lab. which has two alternative laser sources of He-Ne(633nm) and Ar ion(514.5-488nm). Microcomputer was programmed to make scanning od the cell table in X-Y direction and to get the signal through A/D converter. The data points thus obtained are 10000-30000 in number. Using sigmoidal calibration curve, the photoacoustic signal was converted to the amount og the rarget material (human <lambda>-type L-chain). the setting of the accumulating area and the classigication of the amount by coloe were also possible by this new apparatus.2)Development of new immunoassay(1)photoacounstic imaging immunoassayImmuno-enzyme reaction was used for staining. The cross sections of human embryo tissues (spleen and pancreas(5 um thick) were analyzed with 40 <micrn>m resolution. The local and total amounts of the <lambda>-type L-chain were estimated.(2)Multi-item photoacoustic immunoassayHuman <lambda>-type L-chain,<kappa>-type L-chain, and igG were analyzed by the multi-item immuno-reaction test paper which was developed by this study. The determination range was 100nm/ml-100ug/ml. the kit system for this method are under the investigation.3)New Study Field Incidentally Occurred by This ResearchUsing this apparatus. X-ray photoacoustic imaging analysis was developed. This method will open the new field of 3-dimensional photoacoustic mictoanalysis.