Development of X-ray, Laser Photoacoustic Microscopy and Microanalysis

研制了一种新的显微镜，利用聚焦X射线和激光束对样品进行扫描，同时获得光声信号。同步辐射被用作X射线源。为了获得高的空间分辨率，提高了X射线光声探测的灵敏度和测量系统的噪声水平。在Cu、Ni等元素的X射线能量上和能量下分别作减影像，得到各元素的高分辨像。将激光光声显微镜应用于人胎胰腺组织切片中bence-jones蛋白弥散的imacina分析，将两种显微系统合二为一。研制了一种具有两个窗口（一个用于X射线，另一个用于激光）的特殊单元的新型微镜，在研究过程中，用监视摄像机发现了X射线发光。从这一发现开始，视频显微图像分析也得到了发展，并应用于活细胞的分析。

A new microscopy which scans focused X-ray and laser bean, on the sample was developed to get photoacoustic sional simultaneously. Synchrotron radiation was utilized as X-ray source. In order to aet hidh spatial resolution, sensitivity of X-ray photoacoustic detection and noise level of measurement system were improved. The subtraction image at the X-ray enermies above and below Cu, Ni k-edoe was obtained to get hiqhliohted redon of each species. Laser pohotoacoustic microscopy was applied to the imacina analysis of dispersion of the bence-jones protein in human fetal pancreas tissue-sections.Combining the two microscopic systems into one system. which has a special cell with two windows (one for X-ray and another for laser beam), new microsconv-was developed.On the course of this study, X-ray luminescence was found by using monitor video camera. Startine from this founding, video microsconic imaoe analysis was also developed and andlied to the analysis of living cells.