Analysis in Microregion by Fourier Transform Infrared/ Photoacoustic Microscopy

1)傅里叶变换红外光声显微镜装置研制了一台脉冲台上有动镜的干涉仪，用He-Ne激光器和白色光检测动镜的位置。采用步进积分法得到干涉图。微机进行数据处理，包括FFT。聚焦系统采用抛物面镜和椭球面镜，得到1/4倍光源的点像。插入插图以获得更高分辨率。2）光散射样品的非破坏性分析利用该装置对天然药物、胆结石、头发、药片等生物样品进行了测量。天然药物黄柏的光谱表明，样品中主要含有纤维素和小檗碱。胆结石微区的光谱与胆固醇的光谱相似。3）定量微区分析的基础研究进行了稳定He-Ne激光微区分析的基础研究。有人发现，定量微量分析在低至40<mu>米的分辨率是可能的，使用模型染料斑点。本研究应用于组织中生物成分的分析。4）本研究偶然发现的新的研究领域使用本研究开发的高灵敏度光声池，发现使用同步辐射源检测X射线光声效应，这些结果在会议、期刊和一本书中报道。

1) Instrumentation for FT-IR/Photoacoustic MicroscopyAn interferometer was made which had a moving mirror on a pulse stage and had a He-Ne laser and white light for the detection of the location of the moving mirror. Step and integrating method was adopted to get interferogram. Microcomputer was used for data processing which include FFT. For the focusing system, a paraboloid and an ellipsoid mirrors were used to get 1/4 times point image of light source. Apertures were inserted for more resolution. Commercial interferometer was also used for good reproducibility.2) Non-destructive Analysis of Light-Scattering SamplesSeveral types of biological samples, e.g. natural drugs, gallstones, hair,and tablets, were measured by this apparatus. The spectra of the raw bark of a natural drug of Phellodendri Cortex showed that cellulose and Berberine were included principally in the sample. A spectrum of a microregion of a gallstone was similar to that of Cholesterol. The microregions of various raw samples were found to be analyzed by this method.3) Fundamental Study for Quantitative MicroanalysisFundamental study of microanalysis with stable He-Ne laser was performed. It was found that the quantitative microanalysis at down to 40 <mu> m resolution was possible using model dye spots. This study was applied to the analysis of biological components in tissues.4) New Study Field Incidentally Found by This ResearchUsing high sensitive photoacoustic cell which was developed for this study, X-ray photoacoustic effect was found to be detected using synchrotron radiation source.These results were reported in the meetings, journals and a book.