Analysis in Microregion by Fourier Transform Infrared/ Photoacoustic Microscopy

1) FT-IR/光声显微镜干涉仪，在脉冲台上有一个运动反射镜，用He-Ne激光器和白光检测运动反射镜的位置。采用分步积分法得到干涉图。采用微机进行数据处理，其中包括FFT。聚焦系统采用抛物面镜和椭球镜，获得光源1/4倍点像。为了获得更高的分辨率，我们插入了光圈。还使用了商用干涉仪，重现性好。2)光散射样品的无损分析本仪器可检测多种生物样品，如天然药物、胆结石、毛发、片剂等。天然药物黄柏树皮的光谱分析表明，样品中主要含有纤维素和小檗碱。胆结石微区光谱与胆固醇相似。用该方法分析了各种原料样品的微区。3)微量定量分析的基础研究进行了稳定氦氖激光微量分析的基础研究。结果表明，利用模型染料斑点可以进行低至40 <mu> m分辨率的定量微量分析。本研究应用于组织中生物成分的分析。4)偶然发现的新研究领域利用为本研究研制的高灵敏度光声电池，利用同步辐射源探测x射线光声效应。这些结果发表在会议、期刊和一本书中。

1) Instrumentation for FT-IR/Photoacoustic MicroscopyAn interferometer was made which had a moving mirror on a pulse stage and had a He-Ne laser and white light for the detection of the location of the moving mirror. Step and integrating method was adopted to get interferogram. Microcomputer was used for data processing which include FFT. For the focusing system, a paraboloid and an ellipsoid mirrors were used to get 1/4 times point image of light source. Apertures were inserted for more resolution. Commercial interferometer was also used for good reproducibility.2) Non-destructive Analysis of Light-Scattering SamplesSeveral types of biological samples, e.g. natural drugs, gallstones, hair,and tablets, were measured by this apparatus. The spectra of the raw bark of a natural drug of Phellodendri Cortex showed that cellulose and Berberine were included principally in the sample. A spectrum of a microregion of a gallstone was similar to that of Cholesterol. The microregions of various raw samples were found to be analyzed by this method.3) Fundamental Study for Quantitative MicroanalysisFundamental study of microanalysis with stable He-Ne laser was performed. It was found that the quantitative microanalysis at down to 40 <mu> m resolution was possible using model dye spots. This study was applied to the analysis of biological components in tissues.4) New Study Field Incidentally Found by This ResearchUsing high sensitive photoacoustic cell which was developed for this study, X-ray photoacoustic effect was found to be detected using synchrotron radiation source.These results were reported in the meetings, journals and a book.