Combined investigations on the temperature and flow field on an acoustically-driven microfluidic 2D-single cell per well analysis system

该项目的目的是表征基于表面声波（SAW）的二维单孔微流体分析系统。首次使用具有高时空分辨率的测量技术分析细胞（模型粒子）的位置、周围的流场和温度场。这样，就可以研究高频声波（bbb100 MHz）对细胞的影响。利用激光多普勒振动仪对声表面波分布进行了实验表征，利用散光粒子跟踪技术对声表面波的速度场和温度场进行了实验表征，分析了潜在的流体力学和热力学现象，有助于设计可靠的细胞分析系统并建立数值模型进行模拟。最后，细胞上的机械和热应力将被导出，以使长期的生物学实验不损害细胞。然而，在这个项目的过程中，重点是物理现象，不使用活细胞，以确保可重复性，避免使用生物样品带来的额外复杂性。

The aim of the project is the characterization of a 2D single cell per well microfluidic analysis system based on surface acoustic waves (SAW). For the first time, the position of the cell (model particle), the surrounding flow field as well as temperature field will be analysed with measurement techniques featuring high spatial and temporal resolution. In that way, the effects of high frequency acoustic waves (> 100 MHz) on the cells can be investigated. The experimental characterization of the SAW distribution by laser Doppler vibrometry and the velocity and temperature field by astigmatism particle tracking allows to analyse the underlying fluid mechanical and thermo dynamical phenomena, in order to help for the design of reliable cell analysis systems and establish numerical models for their simulation. Finally, the mechanical and thermal stress on the cells will be derived to enable long term experiments in biology without damage on the cells. However, during the course of this project the focus is on the physical phenomena and no living cells will be used, to ensure repeatability and avoid additional complexities from the use of biological samples.