Combined investigations on the temperature and flow field on an acoustically-driven microfluidic 2D-single cell per well analysis system

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The aim of the project is the characterization of a 2D single cell per well microfluidic analysis system based on surface acoustic waves (SAW). For the first time, the position of the cell (model particle), the surrounding flow field as well as temperature field will be analysed with measurement techniques featuring high spatial and temporal resolution. In that way, the effects of high frequency acoustic waves (> 100 MHz) on the cells can be investigated. The experimental characterization of the SAW distribution by laser Doppler vibrometry and the velocity and temperature field by astigmatism particle tracking allows to analyse the underlying fluid mechanical and thermo dynamical phenomena, in order to help for the design of reliable cell analysis systems and establish numerical models for their simulation. Finally, the mechanical and thermal stress on the cells will be derived to enable long term experiments in biology without damage on the cells. However, during the course of this project the focus is on the physical phenomena and no living cells will be used, to ensure repeatability and avoid additional complexities from the use of biological samples.