Combined investigations on the temperature and flow field on an acoustically-driven microfluidic 2D-single cell per well analysis system

该项目的目标是基于声表面波(SAW)的二维单电池每井微流控分析系统的特征。首次使用具有高空间和时间分辨率的测量技术来分析单元(模型粒子)的位置、周围的流场以及温度场。这样，就可以研究高频声波(100 MHz)对细胞的影响。用激光多普勒测振仪测量声表面波分布，用像散粒子跟踪测量速度场和温度场，可以分析潜在的流体力学和热力学现象，从而有助于设计可靠的单元分析系统，并建立数值模型进行模拟。最后，将推导出细胞上的机械和热应力，以便在不损害细胞的情况下进行长期的生物学实验。然而，在这个项目的过程中，重点是物理现象，不会使用活细胞，以确保重复性，并避免使用生物样本带来的额外复杂性。

The aim of the project is the characterization of a 2D single cell per well microfluidic analysis system based on surface acoustic waves (SAW). For the first time, the position of the cell (model particle), the surrounding flow field as well as temperature field will be analysed with measurement techniques featuring high spatial and temporal resolution. In that way, the effects of high frequency acoustic waves (> 100 MHz) on the cells can be investigated. The experimental characterization of the SAW distribution by laser Doppler vibrometry and the velocity and temperature field by astigmatism particle tracking allows to analyse the underlying fluid mechanical and thermo dynamical phenomena, in order to help for the design of reliable cell analysis systems and establish numerical models for their simulation. Finally, the mechanical and thermal stress on the cells will be derived to enable long term experiments in biology without damage on the cells. However, during the course of this project the focus is on the physical phenomena and no living cells will be used, to ensure repeatability and avoid additional complexities from the use of biological samples.