Combined investigations on the temperature and flow field on an acoustically-driven microfluidic 2D-single cell per well analysis system

声驱动微流控二维单细胞每孔分析系统的温度和流场联合研究

• 批准号: 417890455
• 负责人: Professor Dr.-Ing. Christian Cierpka
• 金额: --
• 依托单位: Leibniz-Institut für Festkörper- und Werkstoffforschung Dresden (IFW) e.V.
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/417890455?language=en
• 关键词: Combined; investigations; temperature; flow; field

The aim of the project is the characterization of a 2D single cell per well microfluidic analysis system based on surface acoustic waves (SAW). For the first time, the position of the cell (model particle), the surrounding flow field as well as temperature field will be analysed with measurement techniques featuring high spatial and temporal resolution. In that way, the effects of high frequency acoustic waves (> 100 MHz) on the cells can be investigated. The experimental characterization of the SAW distribution by laser Doppler vibrometry and the velocity and temperature field by astigmatism particle tracking allows to analyse the underlying fluid mechanical and thermo dynamical phenomena, in order to help for the design of reliable cell analysis systems and establish numerical models for their simulation. Finally, the mechanical and thermal stress on the cells will be derived to enable long term experiments in biology without damage on the cells. However, during the course of this project the focus is on the physical phenomena and no living cells will be used, to ensure repeatability and avoid additional complexities from the use of biological samples.

该项目的目的是表征基于表面声波 (SAW) 的每孔 2D 单细胞微流体分析系统。首次采用高空间和时间分辨率的测量技术来分析细胞（模型粒子）的位置、周围的流场以及温度场。通过这种方式，可以研究高频声波（> 100 MHz）对细胞的影响。通过激光多普勒测振法​​对 SAW 分布进行实验表征，通过像散粒子跟踪对速度场和温度场进行实验表征，可以分析潜在的流体力学和热力学现象，以帮助设计可靠的细胞分析系统并为其模拟建立数值模型。最后，将导出细胞上的机械和热应力，以便在不损害细胞的情况下进行长期生物学实验。然而，在该项目过程中，重点是物理现象，不会使用活细胞，以确保可重复性并避免使用生物样品带来的额外复杂性。