新しい染色体解析法(CGH法)を用いた胃癌の染色体異常の解析と遺伝子診断への応用

新染色体分析方法（CGH法）分析胃癌染色体异常及其在基因诊断中的应用

• 批准号: 09770948
• 负责人: 阪倉 長平
• 金额: $ 1.41万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09770948/
• 关键词: CGH; 染色体異常; 遺伝子増幅; 胃癌

前年度までに、我々はCGH法を標準化し、食道癌、胃癌、胃平滑筋肉腫、大腸癌、転移性肝癌、肝細胞癌など各種消化器癌の染色体遺伝子異常の解析を行なった。今後、さらに症例数を増やして、個々の癌の染色体遺伝子診断に有用な病型特異的ゲノム異常を検索した。これまでの胃癌臨床検体72例の解析結果では、既知の変化とは別に、8p23.1をはじめ現在のところ標的候補遺伝子が明らかでない高頻度の変化を示す染色体領域が複数の箇所で同定された(Sakakura et al.,Genes Chromsom Cancer in press)。これらの領域には増幅変化によって胃癌の発症や進展に機能する遺伝子が存在するものと予想される。このうち8p23.1の新規増幅領域に関しては、ハイブリッドセレクションを行い、新規増幅遺伝子を単離した(Sakabe et al.,Cancer Res., in press)。これはhelix-loop-helix構造を有し、IGFと約60%のホモロジーを有していた。また8p23.1以外の新規増幅領域の中には、既知の遺伝子で未だ消化器癌への関与が報告されていない細胞周期調節やシグナル伝達に関係した遺伝子(AIB1,BTAK,AUR 1など)を含むものもあった。これらの遺伝子の増幅や発現を検索し、臨床病理学的因子との関連を調べたところ有意にリンパ節転移、肝転移、予後に差が認められた。AIB1を含むエストロゲンレセプターシグナル伝達系とBTAKによるanuploidy発生が胃癌の悪性化に関与している可能性が示唆された。

In the previous year, we studied the standardization of CGH method, esophageal cancer, gastric cancer, gastric smooth muscle meat, colorectal cancer, metastatic liver cancer, liver cancer cell cancer. In the future, the number of cases of atypical dysentery has been reduced, and the chromosomal abnormalities of two cancers have been cut off to check the number of cases of atypical dysentery. The clinical data of 72 patients with gastric cancer were analyzed. The results showed that the results of 72 patients with gastric cancer were analyzed, the results showed that the patients with gastric cancer had the same diagnosis (Sakakura et al.,Genes Chromsom Cancer in press) in the chromosomal field, the results of 72 patients with gastric cancer were analyzed, the results showed that the patients with gastric cancer had the same diagnosis (Sakakura et al.,Genes Chromsom Cancer in press) in the chromosomal field. In the field of medical treatment, it is possible to improve the diagnosis and treatment of gastric cancer. There is a risk factor in the diagnosis of gastric cancer. The scope of the new rules and regulations (8p23.1) is different from each other, the number of new regulations is different, and that of the new rules is different (Sakabe et al.,Cancer Res., in press). About 60% of the total number of people in the helix-loop-helix market and 60% in the IGF market are in the market. In the field of new regulations other than 8p23.1, it is known that there is no digestive tract cancer in the field, and there is a report that the cell cycle is different from that of the digestive system. There is an error in the cell cycle of the digestive system in the field of new regulations other than 8p23.1 (AIB1,BTAK,AUR 1). The cause of the disease is that the factors of the pathology of the bed are affected by the movement of the liver, the liver, and the pathological factors of the bed. AIB1 is characterized by BTAK, anuploidy, gastric cancer, sexualization, and possibility.

项目成果

Sakakura,C et al.: "Increased apoptosis rats by hyperthermochemoradio therapy for advanced rectal cancers" Surgery Today. 27. 773-776 (1997)

Sakakura,C 等人：“晚期直肠癌的高温放化疗增加了大鼠的细胞凋亡”《今日外科》。

Sakakura, C et al: "Gains, Losses and Amplification of Genomic Materials in Primary Gastric Cancers Anclyged by Comparative Genomic Hybridization" Geres Chromosomes & Cancer. (in press).

Sakakura, C 等人：“比较基因组杂交促进原发性胃癌基因组物质的增加、丢失和扩增”Geres 染色体

Sakakura, C et al: "Chromosomal abeuations in human hepatocellular corcinomas associated with hepatitis C infection by comparative geromic hybridization" Brital Journal of Cancer. (in press).

Sakakura, C 等人：“通过比较基因组杂交研究与丙型肝炎感染相关的人肝细胞癌的染色体畸变”《英国癌症杂志》。

Sakakura C et al: "Overexpression of bax sensitizes breast cancer MCF-7 cells to cisplatin and etoposid" Surgery Today. 27. 676-679 (1997)

Sakakura C 等人：“bax 的过度表达使乳腺癌 MCF-7 细胞对顺铂和依托泊苷敏感”《今日外科》。