High pressure liquid chromatograph coupled with Tandem ion trap and time flow mass detector

高压液相色谱仪与串联离子阱和时间流质量检测器联用

• 批准号: 420757106
• 金额: --
• 依托单位: Brandenburgische Technische Universität Cottbus-Senftenberg (BTU)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/420757106?language=en
• 关键词: pressure; liquid; chromatograph; coupled; Tandem

The recent research becomes more and more focused to organic trace contaminants and in particular to its effects to health and environment. Trace concentrations of pesticides and pharmaceuticals have been found in all environmental compartments including food products, drinking water and human tissues. Effects have been described at ecosystem level, especially in aquatic organisms, but also already in vertebrates - and human cells. Particularly worrisome are synergistic effects of substances occurring at individual concentrations far less known effect concentrations. However, the effects of such trace substances are often unknown or can not be studied properly so far. Therefore, the newly-founded Department 2 of the BTU has defined the research on environmental behavior and health effects of these compounds as one of its main research topics. The behavior of many trace substances in the environment is not or not completely understood so far. Metabolites and degradation products have been sometimes analyzed, but its role for environmental and health effects are in most cases unclear. This is often caused by lacking efficient analytical methods to follow degradation mechanisms at trace concentrations. The LS "Biotechnology of water treatment", is engaged e.g. in the investigation of the environmental behavior of organic phosphorus compounds. The total herbicide glyphosate is a well known representative of this group, that is currently subject of the public debate. Although such phosphonates are produced in large quantities not only for agricultural, but also for industrial and in particular for household applications, where is only very little known about its behavior in the environment and especially about its degradation. One reason for that lack in knowledge is the complicated analytics, which is only available by LC/MS / MS or LC/ICP-MS and is recently done in only a few laboratories in Germany. The identification of many metabolites fails so far due to the lack of suitable equipment and methods. The LC/MS-IT-TOF instrument combines the high sensitivity of the ion-trap for the first time with the mass accuracy of the time-flow technique. This device offers new possibilities for the identification of trace substances and is an important complement to existing techniques such as GC/MS, LC/MS and NMR and highly efficient methods and procedures for the enrichment of organic compounds. With the help of this new technique, new insights into environmental behavior and effects of trace substances may be obtained, which so far are not or not efficiently analyzed.This new equipment will be used also to further link environmental and health research.

近年来，对有机微量污染物的研究越来越受到重视，特别是对其对健康和环境的影响。在包括食品、饮用水和人体组织在内的所有环境隔间中都发现了微量浓度的农药和药物。已经在生态系统水平上描述了这种影响，特别是在水生生物中，但也已经在脊椎动物和人类细胞中描述了这种影响。尤其令人担忧的是，物质在个体浓度下产生的协同效应远低于已知的效应浓度。然而，这些微量物质的影响往往是未知的，或者到目前为止还不能进行适当的研究。因此，新成立的BTU 2部门将这些化合物的环境行为和健康影响的研究确定为其主要研究课题之一。到目前为止，许多微量物质在环境中的行为尚未或尚未完全了解。有时对代谢物和降解产物进行了分析，但在大多数情况下，其对环境和健康影响的作用尚不清楚。这通常是由于缺乏有效的分析方法来跟踪微量浓度下的降解机制。LS“水处理的生物技术”，从事例如有机磷化合物的环境行为的调查。总除草剂草甘膦是这一群体的一个众所周知的代表，目前是公众辩论的主题。虽然这种磷酸盐的大量生产不仅用于农业，而且还用于工业，特别是用于家庭用途，但人们对其在环境中的行为，特别是其降解情况知之甚少。缺乏知识的一个原因是复杂的分析，这只能通过LC/MS /MS或LC/ICP-MS进行，最近在德国只有少数实验室进行。迄今为止，由于缺乏合适的设备和方法，许多代谢物的鉴定失败。LC/MS-IT-TOF仪器首次结合了离子阱的高灵敏度和时间流技术的质量精度。该装置为微量物质的鉴定提供了新的可能性，是对现有技术（如GC/MS、LC/MS和NMR）和有机化合物富集的高效方法和程序的重要补充。在这项新技术的帮助下，可以获得对微量物质的环境行为和影响的新见解，这些都是迄今为止没有或没有有效分析的。这种新设备还将用于进一步将环境和健康研究联系起来。