automated peptide synthesizer

自动化肽合成仪

• 批准号: 423873078
• 金额: --
• 依托单位: Technische Universität Berlin
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/423873078?language=en
• 关键词: automated; peptide; synthesizer

The automated solid phase peptide synthesis is an essential part of experimentally working peptide researcher groups. During the peptide synthesis various peptide chains of different sequence, consisting of amino acids as building blocks are synthetically assembled. The disadvantage of a manual synthesis of peptides are possible errors in the assembly of the sequence, and the restriction of working hours to a normal working day. In constrast, the automated peptide synthesizer does work continously and according to the program without mistakes. Furthermore, though the parallelization of the machine, higher numbers of peptides can be synthesized, as compared to the manual synthesis.The structures of the peptides assembled by an automated peptide synthesized are not only restricted to the 20 proteinogenic amino acids, but also other building blocks can be used for synthesis. In the area of modern peptide research, where higher numbers and amounts of peptides are required, an automated peptide synthesizer is of highest importance in the laboratory.The required machine with the indicated functions is an important complement for research and supports the education and training of doctoral students in a planned research training group (Bioactive Peptides - Innovative Aspects of Synthesis and Biosynthesis). The machine and the established technology will be made accessible to all members of the RTG. Background of this application is a high demand of synthetic peptides serving as peptide substrates on biochemical reactions, of for structural biology investigations, biophysical characterizations and as a starting material for an extended chemical synthesis.

自动化固相肽合成是肽研究小组实验工作的重要组成部分。在肽合成期间，合成组装由氨基酸作为结构单元组成的不同序列的各种肽链。人工合成肽的缺点是序列组装中可能出现错误，以及工作时间限制为正常工作日。自动化肽合成仪在Tumerast中按照程序连续工作，没有错误。此外，通过机器的并行化，与人工合成相比，可以合成更多数量的肽。由自动化合成的肽组装的肽的结构不仅限于20种蛋白质氨基酸，还可以使用其他构建单元进行合成。在现代肽研究领域，需要更高数量的肽，自动化肽合成仪在实验室中至关重要。所需的具有指定功能的机器是研究的重要补充，并支持计划中的研究培训组（生物活性肽-合成和生物合成的创新方面）的博士生的教育和培训。该机器和已建立的技术将提供给RTG的所有成员。本申请的背景是对合成肽的高需求，这些合成肽用作生化反应中的肽底物、结构生物学研究、生物物理表征以及作为扩展化学合成的起始材料。