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Prediction and Control of New Pandemic Influenza Viruses

新型大流行性流感病毒的预测与控制

基本信息

批准号：
01480103
负责人：
KIDA Hiroshi
金额：
$ 4.29万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480103/
关键词：
Influenza virus Hemagglutinin New pandemic strain Epidemiology Prediction Pig Migrating duck Domestic birds 鴨ブタカモ遺伝子再集合

项目摘要

The H3 hemagglutinins (HA) of influenza virus isolates from domestic ducks in southern China were analyzed antigenically and genetically. The results provided molecular evidence of a role of these birds to introduce avian H3 influenza viruses to pigs as "mixing vessels" where A/Hong Kong/68 (H3N2) strain was produced.Experimental infection of pigs with H1-13 influenza A virus strains isolated from different host species provided the follwing informations ;The cells lining the upper respiratory tract of pig were susceptible not only to the HA of H3 influenza viruses of porcine and human origin but also to that of isolates from feral ducks.Genetic reassortants were produced in upper respiratory tract of pig mixedly infected with influenza viruses of duck and porcine origin.Pigs were susceptible to infection with avian influenza viruses eash know HA subtype. It is thus noted that pigs were also susceptible to H4-13 influenza A viruses which had been recognized only in avian species.Hemagglutination-inhibiting antibodies were not detected in the sera of the pigs infected with the avian influenza viruses. Antibody response was, on the other hand, clearly demonstrated in the sera by ELISA.These findings indicate that influenza viruses of other subtypes than H1-3 arepossible to be introduced into pigs and that seropidemiological survey by HI tests may not provide exact information of infection of pigs with avian influenza viruses.

对我国南方家鸭流感病毒分离株H3血凝素进行了抗原性和遗传学分析。本研究结果为禽流感病毒H3亚型在不同宿主中的传播提供了分子证据，为禽流感病毒H1-13亚型在不同宿主中的传播提供了实验依据。猪上呼吸道上皮细胞不仅对人源和猪源H3流感病毒的血凝素敏感，而且对野鸭分离株的血凝素敏感，在猪上呼吸道上皮细胞中产生了遗传排斥物猪对禽流感病毒HA亚型的敏感性较高。由此可见，猪只对H4-13甲型流感病毒亦有易感性，而H4-13甲型流感病毒只在禽类中发现，但在感染禽流感病毒的猪只的血清中，并没有检测到血凝抑制抗体。这些结果表明，H1-3亚型以外的流感病毒也可能被引入猪体内，而HI试验的血清流行病学调查可能不能提供猪感染禽流感病毒的确切信息。