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Evolution and Prediction of Influenza Viruses

流感病毒的进化和预测

基本信息

批准号：
03041013
负责人：
KIDA Hiroshi
金额：
$ 10.24万
依托单位：
Hokkaido University School of Veterinary Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03041013/
关键词：
New pandemic influenza virus Prediction Migratory duck Nesting point Frozen lake water Perpetuation of virus Evolution Newcastle disease virus インフルエンザウイルスパラミクソウイルス水禽糞便北方圏疫学情報永久凍土

项目摘要

On the basis of the results of epidemiological studies of influenza in birds, animals, and humans, we have proposed the route of introduction of the hemagglutinin(HA) gene of the A/Hong Kong/68(H3N2) strain, i.e., from a H3influenza virus circulating in migratory ducks to domestic ducks in Southern China, then to pigs where genetic reassortment occurred with the H2N2 Asian influenza virus to produce the new pandemic strain, A/Hong Kong/68(H3N2).To provide information on the distribution of influenza A viruses in waterfowls at their nesting points in the northern territory, virological survey was carried out in Alaska in summer of 1991 and 1992. From 2,579 fecal samples of birds comprizing 1,913 ducks, 646 geese, 6 swans, 7 dunlins, and 7 gulls, 75 influenza viruses and 82 paramyxoviruses were isolated. Most of the influenza virus isolates were obtained from those of ducks, consisting of 14 H3N8, 47 H4N6, 1 H8N2, 1 H10N2, 11 H10N7, and 1 H10N9 viruses. The antigenic subtypes and isolation rates of the viruses were different in the nesting points of ducks. In 1992, lake water samples were also collected for virus isolation. Out of 23 water samples two H4N6virus strains were isolated from different lakes. This support the notion of the mechanism of perpetuation of influenza viruses in nature, that viruses shed with feces from ducks and have been preserved in frozen lake water in winter in northern territory.Antigenic analysis of the hemagglutinin(HA) of H3 influenza virus isolates using a panel of monoclonal antibodies showed that they were closely related to that of the A/Hong Kong/68(H3N2) influenza virus. The present results also indicate that influenza viruses are maintained and conserved in duck population in their nesting areas. Continued surveillance of influenza viruses in ducks in their nesting areas must provide useful information for prediction of possible new human pandemic influenza virus strains.

根据禽流感、动物流感和人类流感的流行病学研究结果，我们提出了A/Hong Kong/68（H3 N2）毒株的血凝素（HA）基因导入途径，即，从在候鸟身上流行的H3流感病毒，到华南地区的家鸭，再到猪只，猪只与H2 N2亚洲流感病毒发生基因重组，产生新的大流行病毒株A/Hong Kong/68（H3 N2）。提供甲型流感病毒在北领地筑巢地点的水禽身上的分布资料，1991年和1992年夏天在阿拉斯加进行了病毒学调查。从1,913只鸭、646只鹅、6只天鹅、7只邓林鸟和7只海鸥的2,579份粪便中分离到流感病毒75株和副粘病毒82株。大部分流感病毒分离株来自鸭，包括14株H3 N8、47株H4 N6、1株H8 N2、1株H10 N2、11株H10 N7和1株H10 N9病毒。不同鸭群筑巢点病毒的抗原亚型和分离率不同。1992年，还采集了湖水样本进行病毒分离。从23份水样中分离到两株H4 N6病毒，分别来自不同的湖泊。这支持了流感病毒在自然界中永久存在的机制，即病毒随鸭粪便脱落，并在北领地冬季的冰冻湖水中保存下来。用一组单克隆抗体对H3流感病毒分离株的血凝素（HA）进行抗原性分析，结果表明它们与A/Hong Kong/68（H3 N2）流感病毒的HA密切相关。本研究结果还表明，流感病毒在其筑巢区的鸭种群中保持和保守。继续监测鸭在其筑巢区的流感病毒，必须提供有用的信息，预测可能的新的人类大流行性流感病毒株。