The role of extracellular matrix in growth and differentiation of regenerating axons : histochemical examination using monoclonal antibodies

本研究的目的是检查定位在环层小体内核内的物质，这些物质负责将神经支配轴突分化为感觉末梢。在实验中，猫肠系膜中的环层小体被用作产生单克隆抗体的免疫原性试剂。起初，人们期望可以产生针对内核成分的几种不同的单克隆抗体。然而，在研究过程中，据了解，内核太小，不能提供足够的大量免疫原，以能够产生抗体。由于猫的数量有限，一次实验不可能获得1000个或更多的Pacinian小体，目前已获得两种识别外核物质的抗体（PC13和PC92）：PC13针对外核细胞的胞质基质，PC92针对外核细胞的细胞外基质。除了外核细胞外，PC13似乎还识别存在于外核中的成纤维细胞和巨噬细胞的细胞质，但不识别支配小体的神经纤维的神经束膜细胞。PC13不能与神经束膜细胞结合的事实根本没有预料到，因为外核心细胞被认为是来源于神经束膜细胞，因此与神经束膜细胞非常密切相关。PC92可以与环层小体以外的组织的细胞外基质结合。PC13和PC92的抗原物质分别约为80KDa和50KDa MVV。这些抗原物质的表征尚未成功进行。

This study was planned for the purpose of examining substances which are localized within the inner core of Pacinian corpuscle and which are responsible for making differentiate the innervating axon into a sensory terminal. Pacinian corpuscles in the cat mesentery were used in experiments as immunogenic agents for raising monoclonal antibodies. It was at first expected that several different monoclonal antibodies against the inner core constituents could be raised. However, during the course of study, it was understood that the inner core is too small to provide a large amount of immunogen sufficient for being able to raise antibodies. Owing to the limited number of cats available, it was impossible to get so many Pacinian corpuscles as 1000 or more for one experiment.So far, two antibodies (PC13 and PC92) have been obtained which recognize substances in the outer core : the PCI3 is against the cytoplasmic matrix and the PC92 against the extracellular matrix of the outer core cells. The PC13 appeared to recognize, in addition to the outer core cell, the cytoplasm of fibroblasts and macrophages present in the outer core, but not the perineurial cells of the nerve fiber innervating the corpuscle. The fact that PC13 cannot bind to perineurial cells was not expected at all, because the outer core cells are considered to be derived from and, therefore, very closely related to perineurial cells. The PC92 can bind to the extracellular matrix of the tissues other than Pacinian corpuscles. Antigenic substances to PCI3 and PC92 are approximately 80KDa and 50KDa MVV, respectively. The characterization of these antigenic substances has not been carried out successfully.