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Promotion of brain-and spinal-cord regeneration by transplantation of somatic cells(bone marrow stromal cells and choroid plexus ependymal cells)-mechanisms of rescuing injured neural cells, and perspectives for clinical applications-

体细胞（骨髓基质细胞、脉络丛室管膜细胞）移植促进脑脊髓再生-拯救受损神经细胞的机制及临床应用前景-

基本信息

批准号：
17300110
负责人：
IDE Chizuka
金额：
$ 9.66万
依托单位：
Aino University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

We studied spinal cord regeneration by cell transplantation using those cells that are potentially available for clinical application, such as bone marrow stromal cells (BMSCs) and choroid plexus ependymal cells (CPECs).1. BMSCsAutogeneous BMSCs were injected by lumbar puncture to a patient with spinal cord injury (C4,5) on March 23, 2006. This is the first clinical case of spinal cord injury in which BMSCs were transplanted through cerebrospinal fluid (CSF). The patient has as yet had no harmful side effect, while showing a sign of functional improvement. Next, we studied transplantation of bone marrow-derived mononuclear cells in the rat to explore the possibility of cell therapy without any cell culture process for spinal cord-injured patients immediately after admission to the hospital. The rat showed the reduced cavity formation in the spinal cord, and improved locomotion. HGF was high, and TNF-α was low in concentration in the CFS. A large number of blood vessels and nerve fibers were seen in the cavity wall. These regenerative effects are considered due to trophic factors secreted from mononuclear cells.Next, we discovered that many skeletal muscle cells develop from cultured BMSCs.2. CPECsWe showed that conditioned medium of CPEC culture promotes the survival of neurons, and neurite extension in vitro. The same effects were seen with the conditioned medium of BMSCs. Next, it was revealed that the choroid plexus ependymal cell layer contains neural stem cells. Similarly, the third ventricle ependymal cell layer contains a large number of neural stem cells.3. OthersWe showed that a large number of neural stem cells are generated mainly from central canal following crush injury to the spinal cord. At the same time, bFGF production was promoted in the lesion. The question of why these changes of spinal cord do not contribute to the spinal cord regeneration remains to be resolved.

我们利用骨髓基质细胞（BMSC）和脉络丛室管膜细胞（CPEC）等可能用于临床的细胞，通过细胞移植研究脊髓再生。1．骨髓间充质干细胞 2006年3月23日，通过腰椎穿刺将自体骨髓间充质干细胞注射到脊髓损伤（C4，5）患者体内，这是临床上第一例通过脑脊液（CSF）移植骨髓间充质干细胞的脊髓损伤患者。患者尚未出现有害的副作用，同时显示出功能改善的迹象。接下来，我们研究了大鼠骨髓源性单核细胞的移植，以探讨脊髓损伤患者入院后立即进行无需任何细胞培养过程的细胞治疗的可能性。大鼠的脊髓空洞形成减少，运动能力改善。 CFS 中 HGF 浓度较高，TNF-α 浓度较低。腔壁内可见大量血管和神经纤维。这些再生效应被认为是由于单核细胞分泌的营养因子所致。接下来，我们发现许多骨骼肌细胞是由培养的BMSCs发育而来。2． CPEC 我们表明，CPEC 培养物的条件培养基可促进神经元的存活和体外神经突的延伸。 BMSC 的条件培养基也观察到相同的效果。接下来，研究发现脉络丛室管膜细胞层含有神经干细胞。同样，第三脑室室管膜细胞层也含有大量的神经干细胞。 3．其他我们表明，脊髓挤压损伤后，大量神经干细胞主要由中央管产生。同时，病灶内bFGF的产生得到促进。为什么脊髓的这些变化不促进脊髓再生的问题仍有待解决。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The role of basal lamina in nerve regeneration.

基底层在神经再生中的作用。

DOI：
发表时间：
期刊：
Encyclopedic Reference in Neuroscience (in press)
影响因子：
0
作者：
Li N;Nakamura K;Jiang Y;Tsurui H;Matsuoka S;Abe M;Ohtsuji M;Nishimura H;Kato K;Kawai T;Atsumi T;Koike T;Shirai T;Ueno H;Hirose S.;Ide C.
通讯作者：
Ide C.

The original : Clinical Neuroanatomy, M. J. T. Fitz Gerald and Jean Folan-Curran, W. B. Saunders

原文：《临床神经解剖学》，M. J. T. Fitz Gerald 和 Jean Folan-Curran，W. B. Saunders