Expression of vesicle-associated proteins during the course of differentiation of growth cones into synaptic terminals in nerve regeneration (synaptophysin, synaptotagmin, protein kinase C,etc)

神经再生过程中生长锥分化为突触末梢过程中囊泡相关蛋白的表达（突触素、突触结合蛋白、蛋白激酶C等）

• 批准号: 06454142
• 负责人: IDE Chizuka
• 金额: $ 4.22万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454142/
• 关键词: regenerating axon; growth cone; vesicle associated protein; signal transduction protein; synaptophysin; synaptotagmin; Rab3A; protein kinase C; シナプトブレビン; 神経再生; プロティンキナーゼC; 小胞膜関連蛋白質; シナプシンI; 免疫電顕

Growth cones of regenerating axons contain numerous heterogenous vesicles which are postulated to be partly involved in the supply of membranous components to the surface axolemma needed for the growth cone extension. We examined the localization of four synaptic vesicle-associated proteins (Rab3A,synaptophysin, synapsin-1, and synaptotagmin), and protein kinase C subtypes (alpha, beta, gamma, delta, epsilon and zeta) in regenerating growth cones including axonal sprouts at nodes of Ranvier, and in growth cones of cultured neurons.All these molecules were localized in the axonal sprouts which appeared shortly after injury at nodes of Ranvier and in growth cones of extending axons as well, and in neurite growth cones of cultured neurons.These findings indicate that growth cone vesicles are involved in the supply of membranous components by fusion with the surface axolemma in the manner similar to the exocytosis at synapse, and that protein kinase C functions as a key molecule in intracellular signalling for the growth of axons.

再生轴突的生长锥含有许多异质囊泡，推测这些囊泡部分参与向生长锥延伸所需的表面轴突供应膜成分。我们检查了四种突触小泡相关蛋白（Rab3A、突触素、突触蛋白-1 和突触结合蛋白）和蛋白激酶 C 亚型（α、β、γ、δ、ε 和 zeta）在再生生长锥（包括 Ranvier 节点处的轴突芽）和培养的生长锥中的定位。 所有这些分子都定位于损伤后不久出现在郎飞叶结节处的轴突芽中，以及延伸轴突的生长锥中，以及培养神经元的神经突生长锥中。这些发现表明，生长锥囊泡通过与表面轴膜融合，以类似于突触胞吐作用的方式参与膜成分的供应，并且 蛋白激酶 C 作为轴突生长的细胞内信号传导的关键分子。

项目成果

Uehara, K.: "Localization of Synaptptagmin in the regenerating Sprouts Emanating from the Nodes of Ranvier." Acta.Hisochem.Cytochem.28. 401-407

Uehara, K.：“突触蛋白在朗飞结再生芽中的定位。”

Uehara, K.: "Localization of synaptotagmin in the regenerating・・・" Acta Histochem. Cytochem.28. 401-407 (1995)

Uehara, K.：“突触结合蛋白在再生中的定位……”细胞化学学报.28（1995）。

Uehara, K., et al: "Localization of synaptotagmin in the regenerating sprouts emanating from the nodes of Ranvier." Acta Histochem. Cytochem.28. 401-407 (1995)

Uehara, K. 等人：“突触结合蛋白在朗飞结节发出的再生芽中的定位。”

Ide, C.: "Peripheral nerve regeneration" Neurosci.Res.25. 101-121 (1996)

Ide, C.：“周围神经再生”Neurosci.Res.25。

Ide, C.: Nerve regeneration and functional restoration Nerve regeneration and functional restoration.Nishimura press., 421 (1996)

Ide, C.：神经再生和功能恢复 神经再生和功能恢复。西村出版社，421 (1996)