Expression of vesicle-associated proteins during the course of differentiation of growth cones into synaptic terminals in nerve regeneration (synaptophysin, synaptotagmin, protein kinase C,etc)

再生轴突的生长锥含有许多异质性囊泡，这些囊泡被假定为部分参与向生长锥延伸所需的表面轴膜供应膜组分。我们研究了四种突触囊泡相关蛋白的定位（Rab 3A、突触素、突触素-1和突触结合蛋白）和蛋白激酶C亚型（α、β、γ、δ、β和ζ）在再生生长锥中，包括在朗维尔节处的轴突芽，所有这些分子都定位于损伤后不久出现的轴突芽，这些发现表明，生长锥囊泡参与了膜成分的供应，通过与表面轴膜融合的方式类似于突触的胞吐作用，并且蛋白激酶C作为轴突生长的细胞内信号传导的关键分子。

Growth cones of regenerating axons contain numerous heterogenous vesicles which are postulated to be partly involved in the supply of membranous components to the surface axolemma needed for the growth cone extension. We examined the localization of four synaptic vesicle-associated proteins (Rab3A,synaptophysin, synapsin-1, and synaptotagmin), and protein kinase C subtypes (alpha, beta, gamma, delta, epsilon and zeta) in regenerating growth cones including axonal sprouts at nodes of Ranvier, and in growth cones of cultured neurons.All these molecules were localized in the axonal sprouts which appeared shortly after injury at nodes of Ranvier and in growth cones of extending axons as well, and in neurite growth cones of cultured neurons.These findings indicate that growth cone vesicles are involved in the supply of membranous components by fusion with the surface axolemma in the manner similar to the exocytosis at synapse, and that protein kinase C functions as a key molecule in intracellular signalling for the growth of axons.