Mechanisms of spinal cord regeneration by transplantation of matured animal-derived somatic stem cells

大鼠脊髓在Th 9 -10水平挤压伤。移植培养的骨髓基质细胞（BMSCs）开始后1（组1），2（组2）和4周（组3）损伤。将细胞注射到CSF中。在初次注射后4周固定大鼠。三组BBB评分均显著高于对照组。三组中均可见大量的再生轴突从无星形胶质细胞的损伤处长出。缺乏星形胶质细胞的区域由结缔组织组成。轴突被雪旺细胞包围。骨髓间充质干细胞移植治疗亚急性和慢性脊髓损伤是有效的。将从骨髓中分离的单核细胞注射到脊髓损伤大鼠的CSF中。确保了运动改善和组织修复。培养脉络丛上皮细胞，直接移植于大鼠脊髓损伤处。再生的轴突沿着整合到宿主组织中的移植细胞生长。

The spinal cord was crush-injured at Th9-10 level in rats. Transplantation of cultured bone marrow stromal cells (BMSCs) began 1 (group 1), 2 (group 2), and 4 weeks (group 3) after injury. Cells were injected into the CSF. Rats were fixed at 4 weeks after the initial injection. BBB scores were significantly higher in the three groups than the control. Numerous regenerating axons grew out through the lesion devoid of astrocytes in the three groups. Astrocyte-devoid areas are composed of connective tissues. Axons were surrounded by Schwann cells. BMSC transplantation is effective for subacute and chronic spinal cord injuries. Mononuclear cells isolated from the bone marrow were injected into the CSF of spinal cord-injured rats. Locomotor improvement and tissue repair were secured. Choroid plexus epithelial cells were cultured and transplanted directly into the spinal cord lesion of rats. Regenerating axons grew out along engrafted cells that were integrated into the host tissues.