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Facilitation of central nerve regeneration by choroid plexus ependymal cells

脉络丛室管膜细胞促进中枢神经再生

基本信息

批准号：
12480226
负责人：
IDE Chizuka
金额：
$ 9.47万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

1 The choroid plexus was excised from the rat 4^<th> ventricle, and grafted into the dorsal funiculus of the spinal cord at C2 level of the same strain rat. The choroid plexus ependymal cells tended to detach from the basal laminae. Regenerating axons extended along the surface of such changed ependymal cells. The transganglionic labeling of regenerating axons by HRP injection into the sciatic nerve showed that numerous regenerating axons extended through the lesion, while a few of them elongated further into the host tissue rostal to the lesion. Electrophysiological examination demonstrated that the evoked potentials were obtained at the 5mm rostal to the lesion in the experiment group, but not in the control group.2 The effect of choroid plexus ependymal cells to the neurite extension was examined in vitro. The cocultured dorsal root ganglion neurons extended neurites more extensively than those cultured on the laminin coat, or those cocultured with the astrocyte monolayers. For exam … More ple, the total length of the all neurites per neuron was 258±14, 385±15, 565±12μm in cultures on laminin coat, on astrocyte monolayers and on choroid plexus ependymal cell monolayers, respectively.3 The almost same results were obtained with the cultures of neurons from hippocampus This shows that the choroid plexus ependymal cells have the neurite extension effect on the both CNS- and PNS-derived neurons.4 The choroid plexus ependymal cells produce adhesion molecules such as NCAM, N-cadherin and laminin, and trophic factors such as NT-4, GDNF, bFGF, IGF-1 and IGF-2.5 In situ hybridization revealed that choroid plexus ependymal cells express the genes such as transthyretin, phosphodiesterase 1α, gelsolin, phospholipid transfer protein, apolipoprotein E, APT- binding cassette transporter A8, androgen-inducible aldehyde reductase, Na/sulfate cotransporter SUT-1, and FS88.6 Choroid plexus ependymal cells from GFP-transgenic mice were cultured in monolayer. Such cultured ependymal cells were grafted into the injured spinal cord of the same strain mice. After 1-3 weeks, 5-7% of the grafted cells differentiated into the astrocytes. This suggests that choroid plexus ependymal cells have the possibility to give rise to the neural stem cells in the appropriate environment. Less

1 从大鼠第4脑室切除脉络丛，并将其移植到同种大鼠C2水平的脊髓背索中。脉络丛室管膜细胞倾向于从基底层分离。再生轴突沿着这种变化的室管膜细胞的表面延伸。通过将 HRP 注射到坐骨神经中，对再生轴突进行跨神经节标记，结果表明，许多再生轴突延伸穿过病变部位，而其中一些则进一步伸长到病变部位的宿主组织中。电生理检查显示实验组在距病灶5mm处有诱发电位，而对照组则没有。2体外观察脉络丛室管膜细胞对神经突延伸的影响。共培养的背根神经节神经元比在层粘连蛋白涂层上培养的神经元或与星形胶质细胞单层共培养的神经元更广泛地延伸神经突。例如，在层粘连蛋白涂层、星形胶质细胞单层和脉络丛室管膜细胞单层培养物中，每个神经元的所有神经突的总长度分别为 258±14、385±15、565±12μm。 3 用来自海马的神经元培养物获得了几乎相同的结果。细胞对 CNS 和 PNS 源性神经元均具有神经突延伸作用。 4 脉络丛室管膜细胞产生 NCAM、N-cadherin 和层粘连蛋白等粘附分子，以及 NT-4、GDNF、bFGF、IGF-1 和 IGF-2.5 等营养因子。原位杂交显示脉络丛室管膜细胞细胞表达转甲状腺素蛋白、磷酸二酯酶 1α、凝溶胶蛋白、磷脂转移蛋白、载脂蛋白 E、APT 结合盒转运蛋白 A8、雄激素诱导醛还原酶、Na/硫酸盐协同转运蛋白 SUT-1 和 FS88.6 来自 GFP 转基因的脉络丛室管膜细胞等基因小鼠以单层培养。将这种培养的室管膜细胞移植到同种小鼠的受伤脊髓中。 1-3周后，5-7%的移植细胞分化为星形胶质细胞。这表明脉络丛室管膜细胞有可能在适当的环境中产生神经干细胞。较少的