Development of artificial tubes to be used for repairing a large peripheral nerve gap

首先，我们建立了骨骼肌纤维细胞成分去除后，基底层可以恢复的方法：新鲜肌肉组织用洗涤剂（3%十二烷基硫酸钠，37 ℃，2天）处理。将这种去污剂处理的肌肉组织移植到大鼠坐骨神经中。移植后2周可见大量再生纤维，移植后4周再生纤维明显增多，并延伸至远侧断端。在此基础上，将长的移植物（5 ~ 6cm）移植到兔坐骨神经中。移植后4周，大量再生纤维延伸到远端残端。电生理结果表明，胫骨前肌在运动终板和肌梭处均获得了神经再生。用L-乳酸-己内酯管覆盖移植物对神经再生是有效的。其次，探讨了利用人羊膜细胞外基质作为移植物的可能性。 关于我们 地雷。用0.01%无花果蛋白酶处理人羊膜以去除上皮细胞，并制成薄片（ACS），将其储存在无菌条件（0.1%苯扎溴铵）下。从这些片材，基本上制造了三种不同类型的管;（a）直径为1〜2 mm的管，壁厚为7〜10层，（B）每束直径为0.2mm的薄管，（c）直径为1〜 2 mm的管，另外用DEXON网覆盖。将这些管（1cm）移植到大鼠坐骨神经上，并在移植后2 ~ 4周进行检查。在移植物（a）中观察到良好的神经再生，但在（B）和（c）中没有观察到。电生理检查显示，移植后9个月，胫前肌有良好的神经再支配。这表明ACS在适当的条件下是一种很有前途的周围神经再生移植材料，但其基底膜完全修复的胶原基质的制备方法以及ACS在潮湿条件下的成管方法仍需进一步研究。少

First of all, we have developed the method by which the basal laminae can be restored after cellular components had been removed from the skeletal muscle fiber : the fresh muscle tissue was treated by detergent(3% sodium dodecyl sulfate, 2 days 37 ℃). Such detergent-treated muscle tissues were grafted into the rat sciatic nerve. Many regenerating fibers were observed 2 weeks after grafting, and greatly increased in number, extending into the distal stump 4 weeks after grafting. Then, based on these finding, long grafts(5〜6 cm)were transplanted into the rabbit sciatic nerve. Four weeks after grafting, numerous regenerating fibers extended into the distal stump. Electrophysiology demonstrated that tibialis anterior muscle was reinnervated both at motor endplates and at muscle spindles.The covering of the graft by L-lactic acid caprolacton tube was recognized to be effective for nerve regeneration.Next, the possibility of utilizing the extracellular matrix of human amnion as graft was exa … More mined. The human amnion was treated by 0.01% ficin for removing the epithelial cells, and manufactured into a thin sheet(ACS), which were stored in a sterile condition(0.1% benzalkonium). From these sheets, basically three different types of tubes were manufactured ; (a)tubes 1〜2 mm in diameter, and 7〜10 layers in wall, (b)bundles of thin tubes in 0.2 mm in diameter each, (c)tubes 1〜2mm in diameter with the additional covering by dexon mesh. These tubes(1 cm) were grafted to the rat sciatic nerve, and examined 2〜4 weeks after grafting. Good nerve regeneration was observed in the graft(a), but not in(b)and(c). Electrophysiology showed the good reinnervation occurred in the anterior tibialis muscle 9 months after grafting. This indicates that ACS, when properly manufactured, is a promising material as the graft for the peripheral nerve regeneration.The further study is needed to develop the method by which the collagen matrix is prepared on which the basal laminae can be fully restored, and the way in which the ACS can be rolled into tubes in a wet condition. Less