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MODEL OF NERVE FIBER REGENERATION IN CNS BY EXCIMER LASER

准分子激光中枢神经系统神经纤维再生模型

基本信息

批准号：
02557002
负责人：
IDE Chizuka
金额：
$ 8.45万
依托单位：
KOBE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02557002/
关键词：
excimer laser rat spinal cord CNS regeneration axon myelin sheath guide system dissecting microscope Kr-F 神経再生エキシマレ-ザ-中枢神経脊髄後索錐体路視神経組織修復

项目摘要

Excimer laser is characterized by being able to cut the tissue by photochemical effect without giving any destortions or hazardous thermal damages to the surroundings. This fact indicates that the laser-cut tissuemight be readily repaired.We applied excimer laser to cut the spinal cord in order to examine whether the laser-cut dorsal funiculus can be repaired much better than the spinal cord injured by knife or freezing. In addition, we made a guide system which conducts the laser beam to the dissecting microscope for cutting minute areas under direct observations.The laser is Kr-F laser with the wave length of 248 nm. The energy density per pulse, pulse frequency and irradiation area are 30 mJ/pulse, 5 Hz x 1 sec, 16mm x 0.2mm, respectively. Therefore, the energy density per area is 4.69J/cm^2. The depth of the lesion was about 100mum.Nerve fibers are cut sharply. Glia cells and myelin sheaths are severely damaged in the area adjacent to the lesion. The repair processes are observed during 3-28 days post-lesion. About 1 week post-lesion, non-myelinated axons, growth cones, glia cells and Schwann cells are found in the areas of lesion. Axons are later surrounded by myelin sheaths. Many blood vessels are formed in the lesion.The guide system of excimer laser was made. We are using it to cut confined small areas under dissecting microscope. Altough there are many points to be improved, the guide system of excimer laser is considered to be promising for the experimental model of CNS regeneration.

准分子激光的特点是能够利用光化学效应切割组织，而不会对周围环境造成任何破坏或危险的热损害。我们应用准分子激光对脊髓进行切割，以观察激光切割的背索是否能比刀或冷冻损伤的脊髓修复得更好。此外，我们还制作了一个导向系统，将激光引导到解剖显微镜上，用于直接观察微小区域，激光为Kr-F激光器，波长为248 nm。脉冲能量密度为30mJ/脉冲，脉冲频率为5赫兹×1秒，照射面积为16 mm×0.2 mm。因此，单位面积的能量密度为4.69J/cm~2。病变深度约为100 mm，神经纤维明显切断。邻近病变区域的胶质细胞和髓鞘严重受损。损伤后3-28天观察修复过程。损伤后1周左右，损伤区可见无髓轴突、生长锥体、胶质细胞和雪旺细胞。轴突后来被髓鞘包围。并制作了准分子激光导引系统。我们正在用它在解剖显微镜下切割狭小的区域。尽管有许多地方需要改进，但准分子激光引导系统被认为是很有前途的中枢神经系统再生的实验模型。