Induction of Heat Shock Proteins (HSPs) in the Process of Heat Tolerance

耐热过程中热休克蛋白（HSP）的诱导

• 批准号: 01480134
• 负责人: KOSAKA Mitsuo
• 金额: $ 4.35万
• 依托单位: Institute of Tropical Medicine, Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480134/
• 关键词: heat acclimatization; heat shock proteins (HSPs); SDS-PAGE; cytosol fraction; western blotting method; HSP 70; cold shock proteins (CSPs); Hyperthermic Oncology; HSP70; Hyperthermic Oncology; 暑熱・寒冷順化機序; 温熱感受性と耐熱性; 熱ショック蛋白質(HSPs); 体温調節反応; 急性温

The aim of this study is to identify the inductions of HSPs (heat shock proteins) and CSPs (cold shock proteins) in weak heat-and strong cold-tolerant pika in order to clarify the mechanism of its character of weak heat-tolerance and to examine the physiological correlation between thermotolerance and HSPs, or CSPs (1) HSP 70 in rats and HSP 68 in rabbits were induced by the heat-shock. In pika rabbits, the induction of HSP by the heat-shock was not detected by 10% SDS-PAGE not only in liver tissue but also in those tissues such as kidney, adrenal gland, spleen, brain and skeletal muscle. According to previous research, tissues of liver, kidney, adrenal gland and spleen have relatively large ability of inducing HSP 70 than other tissues in hyperthermia of rat. In further study, Western blotting method with anti-HSP 70 antibody was performed. HSP 70 was detected in liver tissue of all rats under heat shock, but only one showed positive in all pikas. These results suggest that the diffic … More ulty of induction of HSPs somewhat relate no the characteristic of weak heat-tolerance in pika rabbits. (2) Induction of HSP 68 by chronic heat load instead of heat-shock was observed on 10% SDS-PAGE. In the experiment of Western blotting method with anti-HSP 70 antibody, the 68 kD protein responded to anti-HSP 70 antibody but the spot of staining showed smaller M. W. size. This discrepancy may be explained as a gradation of proteins including 68 kD protein. (3) Contrarily in cold-shock experiments, induction of CSP was not identified in rat, rabbit or pika. But one result was found that 32 kD protein in cytosol fraction vanished in one rat after cold-shock. Four kinds of plasma proteins (55, 27, 25, 20 kD) vanished in chipmunk, a hybernator, during and after the period of hybernation has been reported in previous paper. Therefore, the present finding showed the similarity to the previous report of chipmunk and suggests that not only hybernation but also short term of cold-shock may lead to the similar result. Furthermore, physiological significance of HSP and CSP was discussed from the viewpoint of thermal acclimation. Less

本研究的目的是鉴定弱耐热鼠兔和强耐冷鼠兔中HSPs（热休克蛋白）和CSPs（冷休克蛋白）的诱导，以阐明其弱耐热特性的机制，并探讨耐热性与HSPs或CSPs之间的生理相关性（1）热休克诱导大鼠HSP 70和兔HSP 68。在鼠兔中，不仅在肝组织中，而且在肾、肾上腺、脾、脑和骨骼肌等组织中，10％SDS-PAGE均未检测到热休克诱导的HSP。前期研究表明，大鼠热疗时，肝、肾、肾上腺、脾等组织较其他组织具有较强的诱导HSP 70 的能力。在进一步的研究中，使用抗HSP 70抗体进行了Western blotting方法。所有热休克大鼠的肝组织中均检测到HSP 70，但所有鼠兔中只有一种呈阳性。这些结果表明，热休克蛋白诱导的困难程度与鼠兔耐热性差的特点有一定关系。 (2)在10％SDS-PAGE上观察到由慢性热负荷而不是热休克诱导HSP 68。在用抗HSP 70抗体进行Western blotting方法的实验中，68 kD的蛋白对抗HSP 70抗体有反应，但染色斑点显示出较小的M. W.尺寸。这种差异可以解释为包括 68 kD 蛋白质在内的蛋白质分级。 (3)相反，在冷激实验中，在大鼠、兔或鼠兔中未发现CSP的诱导。但一项结果发现，冷休克后，一只大鼠的细胞质部分中的 32 kD 蛋白质消失了。之前的论文报道了冬眠动物花栗鼠在冬眠期间和冬眠之后四种血浆蛋白（55、27、25、20 kD）消失。因此，目前的发现与之前关于花栗鼠的报道有相似之处，并表明不仅冬眠而且短期的冷休克也可能导致类似的结果。此外，从热驯化的角度讨论了HSP和CSP的生理意义。较少的

项目成果

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