Function of cementblast: I vitro study and histopathological study

成骨水泥的功能：体外研究和组织病理学研究

• 批准号: 10671970
• 负责人: HARA Yoshitaka
• 金额: $ 1.73万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671970/
• 关键词: Root resorption; Periodontal ligament tissue; Odontoclast; Histopathological study; COX2; 歯根膜細胞; 歯周組織再生

The present study examines whether periodontal ligament (PDL) tissue is capable of inducing root resorption. The distal root of the rat molar was sectioned with disk at the furcation and the PDL tissue was surgically removed from the root (non-PDL group, n=40). The distal root with the PDL intact was also prepared (PDL-intact group, n=40). The roots were transplanted into the dorsal skin of the rat. On the 1st, 3rd, 5th, 7th, 10th, 14th, 21st or 28th day after transplantation, the roots were removed together with surrounding dorsal subcutaneous tissue and were fixed, decalcified and embedded in paraffin. Serial sections from each block were stained with hematoxylin and eosin or tartrate-resistant acid phosphatase (TRAP) staining to observe odontoclast formation. Cyclooxygenase-2 (COX2) was also immunohistologically detected to observe prostaglandinEィイD22ィエD2 production. On the 7th day after transplantation, multinucleated odontoclasts with TRAP and root resorption were observed in the PDL-intact group. The number of odontoclasts peaked on the l0th day after transplantation. On the other hand, no odontoclastic root resorption was observed in the non-PDL group. COX2-positive cells were observed in PDL tissue during the early experimental stage. These results suggest that PDL tissue is involved in the formation of odontoclasts and root resorption.

本研究探讨牙周膜（PDL）组织是否能够诱导牙根吸收。在分叉处用圆盘将大鼠磨牙的远中根切断，并从根中手术去除PDL组织（非PDL组，n=40）。同时制备牙周膜完整的远中牙根（牙周膜完整组，n=40）。将根移植到大鼠背部皮肤中。于移植后第1、3、5、7、10、14、21、28天，将牙根连同周围背侧皮下组织一起切除，固定、脱钙、石蜡包埋。用苏木精-伊红染色或抗酒石酸酸性磷酸酶（TRAP）染色观察破牙细胞的形成。免疫组化检测环氧合酶-2（COX-2）的表达。移植后第7天，PDL完整组可见多核破牙细胞，伴有TRAP和牙根吸收。破牙细胞数量在移植后第10天达到高峰。另一方面，在非PDL组中没有观察到碎牙根吸收。在实验早期，在PDL组织中观察到COX 2阳性细胞。这些结果表明PDL组织参与了破牙细胞的形成和牙根吸收。

项目成果

Chiaki Shiraishi et al.: "Histopathological study on root resorption in teeth transplanted into rat subcutaneous tissue"The Japanese Journal of Conservative Dentistry. 41 (Autumn Issue). 173 (1998)

Chiaki Shiraishi 等人：“移植到大鼠皮下组织的牙齿牙根吸收的组织病理学研究”日本保守牙科杂志。

白石 千秋 ほか: "ラットにおける皮下移植後に生じた歯根吸収に関する病理組織学的研究" 日本歯科保存学会雑誌. 41巻秋季特別号. 173 (1998)

Chiaki Shiraishi 等人：“大鼠皮下移植后牙根吸收的组织病理学研究”，日本保守牙科学会杂志，第 41 卷，秋季特刊（1998 年）。

白石千秋ほか: "ラットにおける皮下移植後に生じた歯根吸収に関する病理組織学的研究"日本歯科保存学雑誌. 41秋季特別号. 173 (1998)

Chiaki Shiraishi 等人：“大鼠皮下移植后牙根吸收的组织病理学研究”，日本保守牙科杂志 41 秋季特刊（1998 年）。